Lipoarabinomannan-Reactive Human Secretory Immunoglobulin A Responses Induced by Mucosal Bacille Calmette-Guérin Vaccination

• Published in: The Journal of infectious diseases Vol. 187; no. 3; pp. 513 - 517
• Main Authors: Brown, Robin M., Cruz, Orlando, Brennan, Michael, Gennaro, Maria L., Schlesinger, Larry, Skeiky, Yasir A. W., Hoft, Daniel F.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.02.2003; University of Chicago Press; Oxford University Press
• Subjects: Adolescent; Adult; Antibodies; Antigens; Bacteria; Bacterial diseases; BCG Vaccine - immunology; Biological and medical sciences; Enzyme linked immunosorbent assay; Female; Fundamental and applied biological sciences. Psychology; Human bacterial diseases; Humans; Immunoglobulin A - immunology; Infectious diseases; Lipopolysaccharides - immunology; Male; Medical sciences; Microbiology; Middle Aged; Mucosal immunity; Mycobacterium tuberculosis; Reactivity; Tuberculosis; Tuberculosis and atypical mycobacterial infections; Tuberculosis vaccine; Vaccination; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Virology; Human; Immune response; Respiratory disease; Mucosa; Vaccination; BCG; Mycobacterial infection; Infection; Prevention; Immunoprophylaxis; Local immunity; Tuberculosis; Mycobacterium tuberculosis; Mycobacteriales; Bacteriosis; Mycobacteriaceae; Bacteria; Serum; Actinomycetes; Humoral immunity
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1086/368096

The ability of 17 recombinant mycobacterial proteins, native antigen 85 complex, lipoarabinomannan (LAM), and Mycobacterium tuberculosis lysate to detect antibody responses induced by bacille Calmette-Guérin (BCG) vaccination and active tuberculosis infection were studied in enzyme-linked immunosorbent assays. Only LAM-reactive serum immunoglobulin G responses were significantly increased in both BCG-vaccinated patients and patients with active tuberculosis (P<.05), and oral BCG vaccination also induced significant increases in LAM-reactive secretory immunoglobulin A (P<.05). LAM-reactive antibody assays can serve as markers of humoral and mucosal immunity in future trials of BCG and newer attenuated mycobacterial vaccines