Kinase Associated-1 Domains Drive MARK/PAR1 Kinases to Membrane Targets by Binding Acidic Phospholipids

• Published in: Cell Vol. 143; no. 6; pp. 966 - 977
• Main Authors: Moravcevic, Katarina, Mendrola, Jeannine M., Schmitz, Karl R., Wang, Yu-Hsiu, Slochower, David, Janmey, Paul A., Lemmon, Mark A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.12.2010
• Subjects: 60 APPLIED LIFE SCIENCES; Amino Acid Sequence; BASIC BIOLOGICAL SCIENCES; CRYSTALLOGRAPHY; Crystallography, X-Ray; Cyclin-Dependent Kinases - metabolism; DETECTION; HeLa Cells; Humans; MEMBRANES; Models, Molecular; Molecular Sequence Data; NEOPLASMS; PHOSPHOLIPIDS; Phospholipids - metabolism; PHOSPHOTRANSFERASES; Protein Kinases - chemistry; Protein Kinases - genetics; Protein Kinases - metabolism; Protein Structure, Tertiary; Protein-Serine-Threonine Kinases - chemistry; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; PROTEINS; REGULATIONS; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Sequence Alignment; TARGETS; YEASTS
• ISSN: 0092-8674; 1097-4172
• DOI: 10.1016/j.cell.2010.11.028

Phospholipid-binding modules such as PH, C1, and C2 domains play crucial roles in location-dependent regulation of many protein kinases. Here, we identify the KA1 domain (kinase associated-1 domain), found at the C terminus of yeast septin-associated kinases (Kcc4p, Gin4p, and Hsl1p) and human MARK/PAR1 kinases, as a membrane association domain that binds acidic phospholipids. Membrane localization of isolated KA1 domains depends on phosphatidylserine. Using X-ray crystallography, we identified a structurally conserved binding site for anionic phospholipids in KA1 domains from Kcc4p and MARK1. Mutating this site impairs membrane association of both KA1 domains and intact proteins and reveals the importance of phosphatidylserine for bud neck localization of yeast Kcc4p. Our data suggest that KA1 domains contribute to “coincidence detection,” allowing kinases to bind other regulators (such as septins) only at the membrane surface. These findings have important implications for understanding MARK/PAR1 kinases, which are implicated in Alzheimer's disease, cancer, and autism. [Display omitted] [Display omitted] ► A C-terminal domain in septin-associated kinases binds negatively charged lipids ► Crystal structures reveal that this is a kinase associated-1 (KA1) domain ► KA1 domains from yeast and human MARK/PAR1 family kinases bind phosphatidylserine ► Cooperation with adjacent domains targets kinases to specific membrane locations