Comparative Analysis of Fecal Microbiota Composition Between Rheumatoid Arthritis and Osteoarthritis Patients

• Published in: Genes Vol. 10; no. 10; p. 748
• Main Authors: Lee, Jin-Young, Mannaa, Mohamed, Kim, Yunkyung, Kim, Jehun, Kim, Geun-Tae, Seo, Young-Su
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.09.2019; MDPI
• Subjects: Abundance; Aged; Arthritis, Rheumatoid - microbiology; Autoimmune diseases; Autoimmunity; Bacteroides; Comparative analysis; Diabetes; Dysbacteriosis; Endothelium; Fecal microflora; Feces; Feces - microbiology; Female; Gastrointestinal Microbiome - genetics; gut microbiota; Humans; Inflammatory bowel disease; Intestinal microflora; Male; Microbiomes; Microbiota; Middle Aged; Osteoarthritis; Osteoarthritis - microbiology; Prevotella; Rheumatoid arthritis; Rheumatology; RNA, Ribosomal, 16S - genetics; rRNA 16S; Statistical analysis; Taxonomy; United States > US; rheumatoid arthritis; osteoarthritis; gut microbiota
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes10100748

The aim of this study was to investigate differences between the gut microbiota composition in patients with rheumatoid arthritis (RA) and those with osteoarthritis (OA). Stool samples from nine RA patients and nine OA patients were collected, and DNA was extracted. The gut microbiome was assessed using 16S rRNA gene amplicon sequencing. The structures and differences in the gut microbiome between RA and OA were analyzed. The analysis of diversity revealed no differences in the complexity of samples. The RA group had a lower Bacteroidetes: Firmicutes ratio than did the OA group. Lactobacilli and , particularly , were more abundant in the RA than in the OA group, although these differences were not statistically significant. The relative abundance of Bacteroides and was lower in the RA group. At the species level, the abundance of certain bacterial species was significantly lower in the RA group, such as . The microbial properties of the gut differed between RA and OA patients, and the RA dysbiosis revealed results similar to those of other autoimmune diseases, suggesting that a specific gut microbiota pattern is related to autoimmunity.