Competitive uptake of porphyrin and LDL via the LDL receptor in glioma cell lines: flow cytometric analysis

• Published in: Cancer letters Vol. 166; no. 1; pp. 79 - 87
• Main Authors: Shibata, Yasushi, Matsumura, Akira, Yoshida, Fumiyo, Yamamoto, Tetsuya, Nakai, Kei, Nose, Tadao, Sakata, Isao, Nakajima, Susumu
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 10.05.2001; Elsevier
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antineoplastic agents; Binding, Competitive; Biological and medical sciences; Brain tumor; Cell Line; Chemotherapy; Fibroblasts - metabolism; Flow Cytometry; Glioma - metabolism; Humans; Lipoproteins, LDL - metabolism; Low-density lipoprotein; Medical sciences; Pharmacology. Drug treatments; Porphyrin; Porphyrins - metabolism; Rats; Receptors, LDL - immunology; Receptors, LDL - metabolism; Trypsin - chemistry; Tumor Cells, Cultured; Brain tumor; Low-density lipoprotein; Flow cytometry; Porphyrin; Antineoplastic agent; Human; Nervous system diseases; Rat; Rodentia; Malignant tumor; In vitro; Lipoprotein LDL; Competitive fixation; Malignant glioma; Vertebrata; Mammalia; Absorption; Binding capacity; Animal; Central nervous system disease; Established cell line; Tumor cell; Biological receptor
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/S0304-3835(00)00717-5

We examined the simultaneous uptake of porphyrin and (LDL) by four established cell lines of glioma and normal fibroblasts using flow cytometry (FCM). The results indicated porphyrin and LDL showed competitive conjugation with the LDL receptor. These results support the theory of the porphyrin uptake via the LDL receptor.