In vivo anti-arthritic and anti-nociceptive effects of ethanol extract of Moringa oleifera leaves on complete Freund's adjuvant (CFA)-induced arthritis in rats

• Published in: Integrative medicine research Vol. 7; no. 1; pp. 85 - 94
• Main Authors: Mahdi, Harith Jameel, Khan, Nurzalina Abdul Karim, Asmawi, Mohd Zaini Bin, Mahmud, Roziahanim, A/L Murugaiyah, Vikneswaran
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2018; Elsevier; 한국한의학연구원
• Subjects: Anti-nociceptive; Complete Freund's adjuvant (CFA); Moringa and Rheumatoid arthritis (RA); Original; 학제간연구; Moringa and Rheumatoid arthritis (RA); Anti-nociceptive; Complete Freund's adjuvant (CFA)
• ISSN: 2213-4220; 2213-4239
• DOI: 10.1016/j.imr.2017.11.002

The medicinal uses of plants are in many cases based exclusively on traditional knowledge without enough scientific evidences. Different parts of Moringa oleifera were traditionally used for the treatment of wide variety of ailments including arthritis and joints pain. The present study had been designed to evaluate the anti-arthritic and anti-nociceptive activities of ethanol extract of Moringa leaves, this being the most abundant plant part suitable for commercial mass production of botanical medicinal products. Complete Freund's adjuvant (CFA)-induced arthritis in rats was used as disease model. CFA-induced inflammatory paw edema, body weight, arthritic index, X-ray radiography, hematological parameters, and walk track and locomotion analysis were all evaluated for the assessment of disease progression. In addition to that, anti-nociceptive activity was examined at different dose levels in both normal and arthritic-induced rats using Eddy's hot plate and tail flick thermal analgesia. The analysis of various arthritic assessment parameters used in this study revealed that Moringa extract has a considerable effect in preventing development or ameliorate arthritis disease severity. Moreover, the ethanol extract of Moringa leaves revealed significant anti-nociceptive activity at in both normal and CFA-induced arthritis rats in a dose-dependent manner. Ethanol extract of Moringa leaves appears to be a really promising as analgesic and arthritis medication, but a larger and more detailed preclinical and clinical studies especially in human is highly recommended.