Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high‐salinity stresses using a full‐length cDNA microarray

• Published in: The Plant journal : for cell and molecular biology Vol. 31; no. 3; pp. 279 - 292
• Main Authors: Seki, Motoaki, Narusaka, Mari, Ishida, Junko, Nanjo, Tokihiko, Fujita, Miki, Oono, Youko, Kamiya, Asako, Nakajima, Maiko, Enju, Akiko, Sakurai, Tetsuya, Satou, Masakazu, Akiyama, Kenji, Taji, Teruaki, Yamaguchi‐Shinozaki, Kazuko, Carninci, Piero, Kawai, Jun, Hayashizaki, Yoshihide, Shinozaki, Kazuo
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.08.2002; Blackwell Science
• Subjects: abiotic stress; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis thaliana; Biological and medical sciences; cDNA microarray; Cold Temperature; Desiccation; Disasters; full‐length cDNA; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Genes, Plant - genetics; Oligonucleotide Array Sequence Analysis; Plant Diseases - chemically induced; Plant Diseases - genetics; Plant physiology and development; Promoter Regions, Genetic - genetics; RNA, Plant - analysis; RNA, Plant - genetics; Sodium Chloride - pharmacology; Time Factors; Transcriptional Activation; Water and solutes. Absorption, translocation and permeability; Cold; Induction; DNA chip; Gene expression; Salinity; Arabidopsis thaliana; Characterization; Signal transduction; Complementary DNA; Cruciferae; Dicotyledones; Angiospermae; Spermatophyta; Experimental plant; Transcription factor; Drought
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1046/j.1365-313X.2002.01359.x

Summary Full‐length cDNAs are essential for functional analysis of plant genes in the post‐sequencing era of the Arabidopsis genome. Recently, cDNA microarray analysis has been developed for quantitative analysis of global and simultaneous analysis of expression profiles. We have prepared a full‐length cDNA microarray containing ≈7000 independent, full‐length cDNA groups to analyse the expression profiles of genes under drought, cold (low temperature) and high‐salinity stress conditions over time. The transcripts of 53, 277 and 194 genes increased after cold, drought and high‐salinity treatments, respectively, more than fivefold compared with the control genes. We also identified many highly drought‐, cold‐ or high‐salinity‐ stress‐inducible genes. However, we observed strong relationships in the expression of these stress‐responsive genes based on Venn diagram analysis, and found 22 stress‐inducible genes that responded to all three stresses. Several gene groups showing different expression profiles were identified by analysis of their expression patterns during stress‐responsive gene induction. The cold‐inducible genes were classified into at least two gene groups from their expression profiles. DREB1A was included in a group whose expression peaked at 2 h after cold treatment. Among the drought, cold or high‐salinity stress‐inducible genes identified, we found 40 transcription factor genes (corresponding to ≈11% of all stress‐inducible genes identified), suggesting that various transcriptional regulatory mechanisms function in the drought, cold or high‐salinity stress signal transduction pathways.