Differential Sensing of MAP Kinases Using SOX-Peptides

• Published in: Angewandte Chemie (International ed.) Vol. 53; no. 51; pp. 14064 - 14068
• Main Authors: Zamora-Olivares, Diana, Kaoud, Tamer S., Jose, Jiney, Ellington, Andrew, Dalby, Kevin N., Anslyn, Eric V.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 15.12.2014; WILEY‐VCH Verlag; Wiley; Wiley Subscription Services, Inc
• Edition: International ed. in English
• Subjects: biosensors; Chemistry; Chemistry, Multidisciplinary; Chemometrics; Classification; Detection; differential sensing; fluorescent probes; Inhibitors; Kinases; mitogen-activated protein kinases; Mitogen-Activated Protein Kinases - antagonists & inhibitors; Mitogen-Activated Protein Kinases - chemistry; Mitogen-Activated Protein Kinases - metabolism; Molecular Structure; Peptides; Peptides - chemistry; Peptides - pharmacology; Physical Sciences; Proteins; Science & Technology; Sulfonamides - chemistry; Sulfonamides - pharmacology; Support vector machines; differential sensing; MOTIFS; ACTIVATED PROTEIN-KINASE; ARRAY; ASSAY; RECOGNITION; PHOSPHORYLATION; biosensors; SENSORS; fluorescent probes; chemometrics; FLUORESCENT; KINOME; DISCRIMINATION; mitogen-activated protein kinases
• ISSN: 1433-7851; 1521-3773
• DOI: 10.1002/anie.201408256

Five SOX peptides are used to classify the MAPK groups and isoforms thereof using chemometrics. The score plots show excellent classification and accuracy, while support vector machine analysis leads to the quantification of ERK and an ERK inhibitor concentration in kinase mixtures. Examination of the loading plots reveals cross‐reactivity among the peptides, and some unexpected surprises. SOX it to the kinases: Five SOX peptides are used to classify groups of mitogen‐activated protein kinases (MAPK) and their isoforms using chemometrics. The score plots show excellent classification and accuracy, while support vector machine analysis serves to quantify ERK and an ERK inhibitor concentration in kinase mixtures. Examination of the loading plots reveals cross‐reactivity among the peptides, and some unexpected results.