Effect of hydrogen peroxide pretreatment on the structural features and the enzymatic hydrolysis of rice straw

• Published in: Biotechnology and bioengineering Vol. 27; no. 10; p. 1418
• Main Authors: Wei, C.J, Cheng, C.Y
• Format: Journal Article
• Language: English
• Published: United States 01.10.1985
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ENZYMIC ACTIVITY; HYDROGEN PEROXIDE; PAILLE DE RIZ; PAJA DE ARROZ; PEROXIDO DE HIDROGENO; PEROXYDE D'HYDROGENE; RICE STRAW
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/bit.260271005

Assessment was made to evaluate the effect of hydrogen peroxide pretreatment on the change of the structural features and the enzymatic hydrolysis of rice straw. Changes in the lignin content, weight loss, accessibility for Cadoxen, water holding capacity, and crystallinity of straw were measured during pretreatment to express the modification of the lignocellulosic structure of straw. The rates and the extents of enzymatic hydrolysis, cellulase adsorption, and cellobiose accumulation in the initial stage of hydrolysis were determined to study the pretreatment effect on hydrolysis. Pretreatment at 60 degrees C for 5 h in a solution with 1% (w/w) H(2)O(2) and NaOH resulted in 60% delignification, 40% weight loss, a fivefold increase in the accessibility for Cadoxen, an one times increase in the water-holding capacity, and only a slight decrease in crystallinity as compared with that of the untreated straw. Improvement on the pretreatment effect could be made by increasing the initial alkalinity and the pretreatment temperature of hydrogen peroxide solution. A saturated improvement on the structural features was found when the weight ratio of hydrogen peroxide to straw was above 0.25 g H(2)O(2)/g straw in an alkaline H(2)O(2) solution with 1% (w/w) NaOH at 32 degrees C. The initial rates and extents of hydrolysis, cellulase adsorption, and cellobiose accumulation in hydrolysis were enhanced in accordance with the improved structural features of straw pretreated. A four times increase in the extent of the enzymatic hydrolysis of straw for 24 h was attributed to the alkaline hydrogen peroxide pretreatment.