DNA sequence similarity between California isolates of Cryptosporidium parvum

• Published in: Applied and Environmental Microbiology Vol. 64; no. 4; pp. 1584 - 1586
• Main Authors: Pereira, M. das G.C. (University of California, Davis, Tulare, CA.), Atwill, E.R, Crawford, M.R, LeFebvre, R.B
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.04.1998
• Subjects: Animals; Animals, Domestic - parasitology; Animals, Wild - parasitology; Base Sequence; Biological and medical sciences; CALIFORNIA; CALIFORNIE; Cattle - parasitology; CRYPTOSPORIDIUM PARVUM; Cryptosporidium parvum - classification; Cryptosporidium parvum - genetics; Cryptosporidium parvum - isolation & purification; Deoxyribonucleic acid; DNA; DNA Primers - genetics; DNA, Protozoan - genetics; DNA, Protozoan - isolation & purification; Environmental and Public Health Microbiology; Fundamental and applied biological sciences. Psychology; General aspects and techniques; Goats - parasitology; Horses - parasitology; Humans; Microbiology; MOLECULAR SEQUENCE DATA; NUCLEOTIDE SEQUENCE; Parasites; Polymerase Chain Reaction; Protozoa; SECUENCIA NUCLEOTIDICA; Sequence Homology, Nucleic Acid; SEQUENCE NUCLEOTIDIQUE; Sewage - parasitology; Species Specificity; Swine - parasitology; California; United States; North America; America; Human; Protozoa; Apicomplexa; Nucleotide sequence; Sequence alignment; Parasite; Homology; Method; Polymerase chain reaction; Cryptosporidium parvum; Animal; Isolate; Livestock
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.64.4.1584-1586.1998

We evaluated whether nucleic acid amplification with primers specific for Cryptosporidium parvum followed by automated DNA sequence analysis of the PCR amplicons could differentiate between California isolates of C. parvum obtained from livestock, humans, and feral pigs. Almost complete sequence identity existed among the livestock isolates and between the livestock and human isolates. DNA sequences from feral pig isolates differed from those from livestock and humans by 1.0 to 1.2%. The reference sequence obtained by Laxer et al. (M. A. Laxer, B. K. Timblin, and R. J. Patel. Am. J. Trop. Med. Hyg. 45:688-694,1991.) differed from California isolates of C. parvum by 1.8 to 3.2%. These data suggest that DNA sequence analysis of the amplicon of Laxer et al. does not allow for differentiation between various strains of C. parvum or that our collection of isolates obtained from various hosts from across California was limited to one strain of C. parvum