Application of Metabolite-Responsive Biosensors for Plant Natural Products Biosynthesis

• Published in: Biosensors (Basel) Vol. 13; no. 6; p. 633
• Main Authors: Zhang, Jianli, Gong, Xinyu, Gan, Qi, Yan, Yajun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.06.2023; MDPI
• Subjects: Binding sites; Biological Products - metabolism; Biosensing Techniques; biosensor; Biosensors; Biosynthesis; Carbon; Cell growth; Chemical research; Chemical synthesis; E coli; Enzymes; Flavonoids; Genes; Global marketing; Industrial engineering; Manufacturing engineering; Metabolic Engineering; Metabolism; Metabolites; Microorganisms; Natural products; Optimization; Plant metabolites; plant natural products; Plant products; Plants; Production processes; Proteins; Review; Terpenes; China; plant natural products; biosensor; metabolic engineering
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios13060633

Plant natural products (PNPs) have shown various pharmaceutical activities, possessing great potential in global markets. Microbial cell factories (MCFs) provide an economical and sustainable alternative for the synthesis of valuable PNPs compared with traditional approaches. However, the heterologous synthetic pathways always lack native regulatory systems, bringing extra burden to PNPs production. To overcome the challenges, biosensors have been exploited and engineered as powerful tools for establishing artificial regulatory networks to control enzyme expression in response to environments. Here, we reviewed the recent progress involved in the application of biosensors that are responsive to PNPs and their precursors. Specifically, the key roles these biosensors played in PNP synthesis pathways, including isoprenoids, flavonoids, stilbenoids and alkaloids, were discussed in detail.