Isolation of a selenite-reducing and cadmium-resistant bacterium Pseudomonas sp. strain RB for microbial synthesis of CdSe nanoparticles

• Published in: Journal of bioscience and bioengineering Vol. 117; no. 5; pp. 576 - 581
• Main Authors: Ayano, Hiroyuki, Miyake, Masaki, Terasawa, Kanako, Kuroda, Masashi, Soda, Satoshi, Sakaguchi, Toshifumi, Ike, Michihiko
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.05.2014; Elsevier
• Subjects: Biological and medical sciences; Biotechnology; Cadmium - analysis; Cadmium - metabolism; Cadmium - pharmacology; Cadmium Compounds - chemistry; Cadmium Compounds - metabolism; Cadmium selenide; Drug Resistance, Bacterial - drug effects; Elemental selenium; Enrichment culture; Fundamental and applied biological sciences. Psychology; Nanoparticles - chemistry; Nanoparticles - metabolism; Nanoparticles - ultrastructure; Oxidation-Reduction; Pseudomonas - classification; Pseudomonas - drug effects; Pseudomonas - isolation & purification; Pseudomonas - metabolism; Pseudomonas sp. strain RB; Selenious Acid - analysis; Selenious Acid - metabolism; Selenite; Selenium - analysis; Selenium - metabolism; Selenium Compounds - chemistry; Selenium Compounds - metabolism; Soil Microbiology; Cadmium selenide; Selenite; Elemental selenium; Pseudomonas sp. strain RB; Enrichment culture; Pseudomonadales; Cadmium; Nanoparticle; Pseudomonas sp; Pseudomonas; Selenides; Resistance; strain RB; Pseudomonadaceae; Bacteria; Isolation; Microorganism; Selenium; Selenites; Culture
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2013.10.010

Bacteria capable of synthesizing CdSe from selenite and cadmium ion were enriched from a soil sample. After repeated transfer of the soil-derived bacterial cultures to a new medium containing selenite and cadmium ion 42 times (during 360 days), an enrichment culture that can simultaneously remove selenite and cadmium ion (1 mM each) from the liquid phase was obtained. The culture's color became reddish-brown, indicating CdSe nanoparticle production, as confirmed by energy-dispersive x-ray spectra (EDS). As a result of isolation operations, the bacterium that was the most responsible for synthesizing CdSe, named Pseudomonas sp. RB, was obtained. Transmission electron microscopy and EDS revealed that this strain accumulated nanoparticles (10–20 nm) consisting of selenium and cadmium inside and on the cells when cultivated in the same medium for the enrichment culture. This report is the first describing isolation of a selenite-reducing and cadmium-resistant bacterium. It is useful for CdSe nanoparticle synthesis in the simple one-vessel operation.