A Simple ICT-Based Fluorescent Probe for HOCl and Bioimaging Applications
Over the past few decades, drug-induced liver damage (DILI) has become a serious public health problem due to drug abuse. Among multifarious reactive oxygen species, mounting evidence attests that ClO has been used as a potential biomarker in DILI. In this work, a new "turn-on" fluorescent...
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Published in | Biosensors (Basel) Vol. 13; no. 7; p. 744 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
01.07.2023
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | Over the past few decades, drug-induced liver damage (DILI) has become a serious public health problem due to drug abuse. Among multifarious reactive oxygen species, mounting evidence attests that ClO
has been used as a potential biomarker in DILI. In this work, a new "turn-on" fluorescent probe
was designed and synthesized by modifying 4'-hydroxybiphenyl-4-carbonitrile (dye
) with
-dimethylthiocarbamate as a response site for detecting ClO
. Probe
displayed a low detection limit (72 nM), fast response time (30 s), wide pH operating range (6-8), great tissue penetration, large Stokes shift (125 nm) and 291-fold fluorescence enhancement at 475 nm in the mapping of ClO
. Probe
could trace amounts of exogenous and endogenous ClO
with high sensitivity in MCF-7 cells and HeLa cells. Expectantly, the fluoxetine-induced liver injury model is successfully established, and probe
has been used for detecting the fluctuation of ClO
levels in the mouse model of fluoxetine-induced liver injury. All in all, probe
with its high specificity, good biological compatibility and liver tissue penetration ability is expected to assist with the early diagnosis of DILI and the clinical screening of various new drugs. We expect that probe
could be efficiently used as a powerful molecular tool to predict clinical DILI and explore molecular mechanisms between molecules and disease. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2079-6374 2079-6374 |
DOI: | 10.3390/bios13070744 |