Identification of new chalcone synthase genes for flower pigmentation in the Japanese and common morning glories [Ipomoea purpurea]

• Published in: Plant and cell physiology Vol. 38; no. 6; pp. 754 - 758
• Main Authors: Tanaka, S.F. (National Inst. for Basic Biology, Okazaki, Aichi (Japan)), Hoshino, A, Hisatomi, Y, Habu, Y, Hasebe, M, Iida, S
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.06.1997
• Subjects: Acyltransferases - genetics; Base Sequence; Biological and medical sciences; CALCONA; CHALCONE; Chalcone synthase gene; CHALCONES; DNA, Complementary; ESPECE; ESPECIES; Flavonoid; FLEUR; FLORES; Flower pigmentation; FLOWERS; Fundamental and applied biological sciences. Psychology; GENE; Gene expression; GENES; Genes, Plant; IDENTIFICACION; IDENTIFICATION; IPOMOEA; Isoenzymes - genetics; LIASAS; LYASE; LYASES; Metabolism; Molecular Sequence Data; Morning glory; Phylogeny; PIGMENTACION; PIGMENTATION; Pigmentation - genetics; Plant physiology and development; Plants - enzymology; Plants - genetics; Sequence Homology, Amino Acid; SPECIES; Storage and secretion, pigments, phytochrome; Acyltransferases; Flower; Nucleotide sequence; Enzyme; Transferases; Homology; Biosynthesis; Phylogeny; Gene expression; Convolvulaceae; Flavonoid; Pigmentation; Complementary DNA; Naringenin-chalcone synthase; Dicotyledones; Angiospermae; Spermatophyta
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a029232

New cDNA sequences for chalcone synthase (CHS), a key enzyme in the flavonoid biosynthesis, were obtained from the Japanese and common morning glories; they are more closely related to other CHS sequences than the six previously described CHS genes from the same plants. The newly isolated CHS-D gene is abundantly expressed in the pigmented flower buds, while its expression is drastically reduced in the white flower buds. Thus CHS-D appears to produce major CHS transcripts for flower pigmentation.