A fast, comprehensive screening method for doping agents in urine by gas chromatography–triple quadrupole mass spectrometry

• Published in: Journal of Chromatography A Vol. 1218; no. 21; pp. 3306 - 3316
• Main Authors: Van Eenoo, Peter, Van Gansbeke, Wim, De Brabanter, Nik, Deventer, Koen, Delbeke, Frans T.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 27.05.2011; Elsevier
• Subjects: Albuterol - urine; Anabolic steroids; Analysis; beta-adrenergic antagonists; Beta-agonist; Beta-blocker; Biological and medical sciences; Cannabis; Doping; Doping in Sports; Dronabinol - analogs & derivatives; Dronabinol - urine; Drug addictions; Estranes - urine; gas chromatography; Gas Chromatography-Mass Spectrometry - methods; General pharmacology; hormone antagonists; Humans; hydrogen; Least-Squares Analysis; liquid chromatography; mass spectrometry; Medical sciences; Narcotic; narcotics; Performance-Enhancing Substances - urine; Pharmacology. Drug treatments; Reproducibility of Results; screening; sports; steroids; Stimulant; Substance Abuse Detection - methods; Toxicology; Urine; Urine; Stimulant; Cannabis; Narcotic; Doping; Beta-blocker; Anabolic steroids; Beta-agonist; Steroid; Biological fluid; Tandem mass spectrometry; Chemical analysis; Metabolite; Volunteer; Banned substance; β-Adrenergic receptor agonist; Narcotic analgesic; Cannabinoid; Derivatization; Quadrupole spectrometer; β-Adrenergic receptor; Qualitative analysis; Drug of abuse; Antagonist; Quantitative analysis; Human; Validation; Terpenoid; Healthy subject; Coupled method; Elimination; Medical screening; Gas chromatography; Anabolic agent; Beta blocking agent
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2010.09.082

The use of performance enhancing drugs in sports is prohibited. For the detection of misuse of such substances gas chromatography or liquid chromatography coupled to mass spectrometry are the most frequently used detection techniques. In this work the development and validation of a fast gas chromatography tandem mass spectrometric method for the detection of a wide range of doping agents is described. The method can determine 13 endogenous steroids (the steroid profile), 19-norandrosterone, salbutamol and 11-nor-Δ9-tetrahydrocannabinol.9carboxylic acid in the applicable ranges and to detect qualitatively over 140 substances in accordance with the minimum required performance levels of the World Anti-Doping Agency in 1 ml of urine. The classes of substances included in the method are anabolic steroids, β2-agonists, stimulants, narcotics, hormone antagonists and modulators and beta-blockers. Moreover, using a short capillary column and hydrogen as a carrier gas the run time of the method is less than 8 min.