Intrathecal Injection of Autologous Mesenchymal Stem-Cell-Derived Extracellular Vesicles in Spinal Cord Injury: A Feasibility Study in Pigs

• Published in: International journal of molecular sciences Vol. 24; no. 9; p. 8240
• Main Authors: Shulman, Ilya, Ageeva, Tatyana, Kostennikov, Alexander, Ogurcov, Sergei, Tazetdinova, Leysan, Kabdesh, Ilyas, Rogozhin, Alexander, Ganiev, Ilnur, Rizvanov, Albert, Mukhamedshina, Yana
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.05.2023; MDPI
• Subjects: Animals; Axons; Cytochalasin B; Extracellular Vesicles; Feasibility Studies; Health aspects; Hogs; Inclusions; Injections, Spinal; Lipids; Locomotor activity; Membranes; Mesenchymal Stem Cells; Myelination; pig; Proteins; Reperfusion; Spinal Cord; Spinal cord injuries; Spinal Cord Injuries - therapy; spinal cord injury; Stem cell research; Stem cells; Structure-function relationships; Swine; Transplantation; Vesicles; Germany; Russia; spinal cord injury; extracellular vesicles; mesenchymal stem cells; pig
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24098240

Spinal cord injury (SCI) remains one of the current medical and social problems, as it causes deep disability in patients. The use of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) is one strategy for stimulating the post-traumatic recovery of the structure and function of the spinal cord. Here, we chose an optimal method for obtaining cytochalasin B-induced EVs, including steps with active vortex mixing for 60 s and subsequent filtration to remove nuclei and disorganized inclusions. The therapeutic potential of repeated intrathecal injection of autologous MSC-derived EVs in the subacute period of pig contused SCI was also evaluated for the first time. In this study, we observed the partial restoration of locomotor activity by stimulating the remyelination of axons and timely reperfusion of nervous tissue.