The expression and cellular localization of phospholipase D isozymes in the developing mouse testis

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Vol. 8; no. 3; pp. 209 - 212
• Main Authors: Kim, S.J. (Cheju National University, Jeju, Republic of Korea), Kim, H.C. (Cheju National University, Jeju, Republic of Korea), Lee, Y.D. (Cheju National University, Jeju, Republic of Korea), Hyun, J.W. (Cheju National University, Jeju, Republic of Korea), Lee, Y.H. (Chungnam National University, Daejeon, Republic of Korea), Shin, M.K. (Pusan National University, Busan, Republic of Korea), Min, D.S. (Pusan National University, Busan, Republic of Korea), E-mail: minds@pusan.ac.kr, Shin, T.K. (Cheju National University, Jeju, Republic of Korea), E-mail: shint@cheju.ac.kr
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society of Veterinary Science 01.09.2007; 대한수의학회
• Subjects: Animals; biosynthesis; Blotting, Western; cellular localization; enzymology; ESPERMATOGENESIS; Female; germ cells; growth & development; Immunohistochemistry; Isoenzymes; isozymes; Leydig cells; Male; metabolism; MICE; Mice, Inbred BALB C; Original; phospholipase D; Phospholipase D - biosynthesis; Phospholipase D - metabolism; physiology; postnatal development; protein synthesis; RATON; sexual development; SOURIS; SPERMATOGENESE; SPERMATOGENESIS; Spermatogenesis - physiology; TESTES; TESTICULE; TESTICULOS; Testis; Testis - enzymology; Testis - growth & development; Western blotting; 수의학
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2007.8.3.209

To examine the involvement of phospholipase D (PLD) isozymes in postnatal testis development, the expression of PLD1 and PLD2 was examined in the mouse testis at postnatal weeks 1, 2, 4, and 8 using Western blot analysis and immunohistochemistry. The expression of both PLD1 and PLD2 increased gradually with development from postnatal week 1 to 8. Immunohistochemically, PLD immunoreactivity was detected in some germ cells in the testis and interstitial Leydig cells at postnatal week 1. PLD was mainly detected in the spermatocytes and residual bodies of spermatids in the testis after 8 weeks after birth.