Binding specificities of the lectins PNA, WGA and UEA I to polyvinylchloride-adsorbed glycosphingolipids

• Published in: FEBS letters Vol. 205; no. 1; pp. 51 - 55
• Main Authors: Molin, Kent, Fredman, Pam, Svennerholm, Lars
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.09.1986; Elsevier
• Subjects: agglutinins; Arachis; Biological and medical sciences; ELISA; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Ganglioside; Glycosphingolipid; glycosphingolipids; Glycosphingolipids - analysis; III4Fuc-LcOse4Cer (see also table 1); Interactions. Associations; Intermolecular phenomena; Lectin; Lectins; Molecular biophysics; Peanut Agglutinin; Plant Lectins; PNA, peanut agglutinin; Polyvinyl Chloride; Receptors, Mitogen - analysis; UEA I, Ulex europeus agglutinin I. Ganglioside nomenclature according to Svennerholm [1.] Fuc-GM1 and Fuc-nLA1 are fucosylated GM1 (IV2Fuc,II3NeuAc-GgOse4Cer) and nLA1 (III3FucnLcOse4Cer), respectively. 3'-nLM1 and 6'-nLM1 are shorthand designations for IV3NeuAc-nLcOse4Cer and IV6NeuAc-nLcOse4Cer, respectively. Lea is the Lewisa blood group defining antigen; Ulex europeus; WGA, wheat germ agglutinin; wheat germ; Wheat Germ Agglutinins; Lectin; WGA, wheat germ agglutinin; Ganglioside; PNA, peanut agglutinin; Glycosphingolipid; UEA I, Ulex europeus agglutinin I. Ganglioside nomenclature according to Svennerholm [1.] Fuc-GM1 and Fuc-nLA1 are fucosylated GM1 (IV 2Fuc,II 3NeuAc-GgOse 4Cer) and nLA1 (III 3FucnLcOse 4Cer), respectively. 3'-nLM1 and 6'-nLM1 are shorthand designations for IV 3NeuAc-nLcOse 4Cer and IV 6NeuAc-nLcOse 4Cer, respectively. Le a is the Lewis a blood group defining antigen; III 4Fuc-LcOse 4Cer (see also table 1); ELISA; Monocotyledones; Phytoagglutinin; Support; Immobilization; Specificity; Gramineae; Angiospermae; Molecular association; Spermatophyta; Polyvinyl chloride; ELISA assay; Triticum aestivum
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(86)80864-X

The binding specificities of the lectins PNA (peanut agglutinin), WGA (wheat germ agglutinin), and UEA I ( Ulex europeus agglutinin I) against glycosphingolipids were investigated using an enzyme-linked immunosorbent assay (ELISA), utilizing the biotin-avidin system for detection of bound lectin. PNA showed the highest affinity to GA1, but also bound, though less strongly, to GM1 and GD1b. WGA bound to 3'-nLM1 and 6'-nLM1, the former twice as strongly as the latter, but not to any sialic acid containing glycolipid of the gangliotetraose series. UEA I showed a high affinity for the Le a glycolipid which has an α 1-4 linked fucose but not for the glycolipids with αl-3 or α 1–2 linked fucose. Interestingly, 3'-nLM1 and nLA1, glycolipids lacking fucose, also bound UEA I. The results show that lectins should be used with caution for establishing terminal sugar sequences in glycosphingolipids.