Endothelin-1, phorbol esters and phenylephrine stimulate MAP kinase activities in ventricular cardiomyocytes

• Published in: FEBS letters Vol. 317; no. 3; pp. 271 - 275
• Main Authors: Bogoyevitch, Marie A., Glennon, Peter E., Sugden, Peter H.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.02.1993; Elsevier
• Subjects: 0.05% (v/v) Tween-20 in phosphate-buffered saline; Amino Acid Sequence; Animals; Animals, Newborn; Biological and medical sciences; Calcium-Calmodulin-Dependent Protein Kinases; Cardiac hypertrophy; Cardiology. Vascular system; Cardiomegaly - enzymology; cardiomyocytes; Cells, Cultured; Chromatography, Liquid - methods; Cultured neonatal rat ventricular cardiomyocyte; Disease Models, Animal; ECL; Endothelin; endothelin-1; Endothelins - pharmacology; enhanced chemiluminescence; Enzyme Activation; fast protein liquid chromatography; FCS; foetal calf serum; FPLC; Glycogen Synthase Kinase 3; Heart; Heart failure, cardiogenic pulmonary edema, cardiac enlargement; LAR; leukocyte common antigen-related protein tyrosine phosphatase; MAP kinase; MBP; Medical sciences; Mitogen-activated protein kinase; Molecular Sequence Data; myelin basic protein; Myocardium - enzymology; PBST; phenylephrine; Phenylephrine - pharmacology; phenylmethanesulphonyl fluoride; phorbol 12-myristate 13-acetate; phorbol 12-myristate 13-acetate diester; Phorbol ester; Phosphoprotein Phosphatases - metabolism; PKC; PMA; PMSF; PP2A; protein kinase; protein kinase C; Protein Kinases - drug effects; Protein Kinases - metabolism; protein serine/threonine phosphatase 2A; Rats; SDS-PAGE; sodium dodecyl sulphate-polyacrylamide gel electrophoresis; stimulation; TCA; Tetradecanoylphorbol Acetate - pharmacology; trichloroacetic acid; ventricle; α 1-Adrenergic agonist; α1-Adrenergic agonist; FPLC; Endothelin; PMA; Phorbol ester; Mitogen-activated protein kinase; Cardiac hypertrophy; α 1-Adrenergic agonist; MAP kinase; PKC; PP2A; ECL; ET; SDS-PAGE; TCA; PE; PMSF; PBST; FCS; MBP; Cultured neonatal rat ventricular cardiomyocyte; LAR; Heart; Cell culture; Pathophysiology; Rat; Peptide hormone; Rodentia; Cardiovascular disease; Heart ventricle; Endothelin 1; In vitro; Myocyte; Signal transduction; Vertebrata; Mammalia; Enzymatic activity; Animal; Hypertrophy
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(93)81291-7

ET-1 stimulated MBP kinase activity in cultured cardiomyocytes. Maximal activation (3.5-fold) was at 5 min. EC 50 was 0.2 nM. PMA or PE also increased MBP kinase (4- or 2.5-fold, respectively). Pre-treatment with PMA down-regulated the subsequent response to ET-1 or PMA. ET-1- or PMA-stimulated MBP kinase was resolved into 2 major (peaks II and IV) and 2 minor peaks by FPLC on Mono Q. Peaks II and IV were inactivated by either LAR or PP2A. Renatured MBP kinase activities following SDS-PAGE in MBP-containing gels and immunoblot analysis showed that peak II was a p42 MAP kinase and peak IV was a p44 MAP kinase.