Interleukin‐10 plays a key role in the modulation of neutrophils recruitment and lung inflammation during infection by Streptococcus pneumoniae

• Published in: Immunology Vol. 146; no. 1; pp. 100 - 112
• Main Authors: Peñaloza, Hernán F., Nieto, Pamela A., Muñoz‐Durango, Natalia, Salazar‐Echegarai, Francisco J., Torres, Javiera, Parga, María J., Alvarez‐Lobos, Manuel, Riedel, Claudia A., Kalergis, Alexis M., Bueno, Susan M.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.09.2015; Wiley; John Wiley & Sons, Ltd
• Subjects: Animals; bacterial infection; Bacterial Load - genetics; Bacterial Load - immunology; Brain - microbiology; cytokines; Human health and pathology; Inflammation - immunology; Interferon-gamma - biosynthesis; Interleukin-10 - biosynthesis; Interleukin-10 - genetics; Interleukin-10 - immunology; Interleukin-1beta - biosynthesis; Life Sciences; Lung - immunology; Lung - microbiology; Lung - pathology; lung inflammation; Mice; Mice, Inbred C57BL; Mice, Knockout; Neutrophil Infiltration - immunology; neutrophils; Neutrophils - immunology; Original; Pneumonia, Pneumococcal - immunology; Pneumonia, Pneumococcal - mortality; Spleen - microbiology; Streptococcus pneumoniae - immunology; systemic bacterial infection; Tumor Necrosis Factor-alpha - biosynthesis; Tumor Necrosis Factor-alpha - immunology; cytokines; systemic bacterial infection; bacterial infection; neutrophils; lung inflammation; lung inflammation
• ISSN: 0019-2805; 1365-2567
• DOI: 10.1111/imm.12486

Summary Streptococcus pneumoniae is a major aetiological agent of pneumonia worldwide, as well as otitis media, sinusitis, meningitis and sepsis. Recent reports have suggested that inflammation of lungs due to S. pneumoniae infection promotes bacterial dissemination and severe disease. However, the contribution of anti‐inflammatory molecules to the pathogenesis of S. pneumoniae remains unknown. To elucidate whether the production of the anti‐inflammatory cytokine interleukin‐10 (IL‐10) is beneficial or detrimental for the host during pneumococcal pneumonia, we performed S. pneumoniae infections in mice lacking IL‐10 (IL‐10−/− mice). The IL‐10−/− mice showed increased mortality, higher expression of pro‐inflammatory cytokines, and an exacerbated recruitment of neutrophils into the lungs after S. pneumoniae infection. However, IL‐10−/− mice showed significantly lower bacterial loads in lungs, spleen, brain and blood, when compared with mice that produced this cytokine. Our results support the notion that production of IL‐10 during S. pneumoniae infection modulates the expression of pro‐inflammatory cytokines and the infiltration of neutrophils into the lungs. This feature of IL‐10 is important to avoid excessive inflammation of tissues and to improve host survival, even though bacterial dissemination is less efficient in the absence of this cytokine.