An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae)

Premise Seaweeds are gaining substantial research interest, particularly for genomic applications, where high‐quality DNA is a prerequisite. Extracting DNA from these organisms presents challenges due to high levels of biomacromolecules resulting from their diverse cell structures. Existing protocol...

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Published inApplications in plant sciences Vol. 13; no. 1; pp. e11625 - n/a
Main Authors Hossen, Riyad, Courtney, Myles, Sim, Alasdair, Khan, Md Abdullah Al Kamran, Verbruggen, Heroen, Bringloe, Trevor
Format Journal Article
LanguageEnglish
Published United States John Wiley & Sons, Inc 01.01.2025
John Wiley and Sons Inc
Wiley
Subjects
absorbance ratios
Alcohol
Algae
Cetyltrimethylammonium bromide
Deoxyribonucleic acid
Detergents
DNA
enzymes
Ethanol
Genomics
high‐molecular‐weight DNA
macroalgae
macrophyte
marine
Methods
Phenols
Protocol
Protocol Note
seaweed
Seaweeds
Ulvophyceae
United States > US
Massachusetts
California
seaweed
high‐molecular‐weight DNA
macrophyte
absorbance ratios
marine
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Summary:Premise Seaweeds are gaining substantial research interest, particularly for genomic applications, where high‐quality DNA is a prerequisite. Extracting DNA from these organisms presents challenges due to high levels of biomacromolecules resulting from their diverse cell structures. Existing protocols often lack versatility, leading to inconsistent outcomes across various materials and taxa, which highlights the need for a universal method for use with a variety of green seaweed samples. Methods and Results We optimized the conventional cetyltrimethylammonium bromide (CTAB) protocol for green seaweed DNA extraction. Our method, involving an initial sample treatment, lysis buffer adjustment, and enzyme incubation alterations, outperformed the conventional CTAB and commercial kits in terms of DNA yield and purity. Notably, the protocol's effectiveness was demonstrated across various green algal materials and preservation methods, and was tested with downstream applications with satisfactory results. Conclusions Our optimized CTAB protocol offers a reliable solution for high‐quality genomic DNA extraction from a wide variety of green seaweed samples.
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ISSN:2168-0450
2168-0450
DOI:10.1002/aps3.11625