Eutrophic status influences the impact of pesticide mixtures and predation on Daphnia pulex populations

• Published in: Ecology and evolution Vol. 11; no. 9; pp. 4046 - 4057
• Main Authors: Oliveira dos Anjos, Talles Bruno, Polazzo, Francesco, Arenas‐Sánchez, Alba, Cherta, Laura, Ascari, Roberto, Migliorati, Sonia, Vighi, Marco, Rico, Andreu
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.05.2021; John Wiley and Sons Inc; Wiley
• Subjects: Abundance; Agricultural ecosystems; Aquatic ecosystems; Aquatic populations; Availability; Bayesian analysis; Bayesian shrinkage; Biodiversity; Chlorpyrifos; Daphnia pulex; Diuron; Ecological effects; Eutrophic environments; Eutrophication; Evaluation; Experiments; Factorial design; Food availability; Freshwater ecosystems; Herbicides; Insecticides; Mathematical models; multiple stressors; Nutrient availability; Nutrients; Original Research; Pesticides; Population structure; Populations; Predation; Statistical models; Synergistic effect; Trophic levels; Trophic status; predation; eutrophication; pesticides; Bayesian shrinkage; multiple stressors
• ISSN: 2045-7758; 2045-7758
• DOI: 10.1002/ece3.7305

Pesticides, nutrients, and ecological stressors such as competition or predation co‐occur in freshwater ecosystems impacted by agriculture. The extent to which combinations of these stressors affect aquatic populations and the role of nutrients availability in modulating these responses requires further understanding. In this study, we assessed how pesticides affecting different taxonomic groups and predation influence the response of Daphnia pulex populations under different trophic conditions. An outdoor experiment was designed following a factorial design, with the insecticide chlorpyrifos, the herbicide diuron, and the predation by Notonecta sp. individuals as key stressors. The single impact of each of these stressors, and their binary and tertiary combinations, was evaluated on D. pulex abundance and population structure under mesotrophic and eutrophic conditions for 21 days. Data were analyzed using generalized linear mixed models estimated by means of a novel Bayesian shrinkage technique. Our study shows a significant influence of each of the evaluated stressors on D. pulex abundance; however, the impacts of the herbicide and predation were lower under eutrophic conditions as compared to the mesotrophic ones. We found that binary stressor interactions were generally additive in the mesotrophic scenario, except for the herbicide–predation combination, which resulted in synergistic effects. The impacts of the binary stressor combinations in the eutrophic scenario were classified as antagonistic, except for the insecticide–herbicide combination, which was additive. The tertiary interaction resulted in significant effects on some sampling dates; however, these were rather antagonistic and resembled the most important binary stressor combination in each trophic scenario. Our study shows that the impact of pesticides on freshwater populations depends on the predation pressure, and demonstrates that the combined effect of pesticides and ecological stressors is influenced by the food availability and organism fitness related to the trophic status of freshwater ecosystems. Our study shows that the impact of pesticides on Daphnia pulex populations is influenced by predation. Moreover, it demonstrates that the combined effect of pesticides and ecological stressors is influenced by food availability and organism fitness related to the trophic status of freshwater ecosystems. Therefore, this study supports the incorporation of ecological interactions and trophic status as key parameters for the risk assessment of pesticides.