Nucleotide sequence and organization of potato leafroll virus genomic RNA

• Published in: FEBS letters Vol. 245; no. 1; pp. 51 - 56
• Main Authors: van der Wilk, Frank, Huisman, Marianne J., Cornelissen, Ben J.C., Huttinga, Harm, Goldbach, Rob
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 13.03.1989
• Subjects: Amino Acid Sequence; Amino acid sequence homology; Base Sequence; BWYV, beet western yellows virus; BYDV, barley yellow dwarf virus; Capsid - genetics; CarMV, carnation mottle virus; cDNA cloning; Codon; CPMV, cowpea mosaic virus; DNA - genetics; Laboratorium voor Virologie; Laboratory of Virology; Molecular Sequence Data; Molecular Weight; Nucleotide sequence; ORF, open reading frame; Plant Viruses - genetics; PLRV, potato leafroll virus; Potato leafroll virus; Protein Biosynthesis; RNA virus, plant positive-strand; RNA, Viral - genetics; RNA-Directed DNA Polymerase - genetics; Sequence Homology, Nucleic Acid; Viral Proteins - genetics; RNA virus, plant positive-strand; BWYV, beet western yellows virus; CPMV, cowpea mosaic virus; cDNA cloning; Nucleotide sequence; Amino acid sequence homology; BYDV, barley yellow dwarf virus; Potato leafroll virus; ORF, open reading frame; PLRV, potato leafroll virus; CarMV, carnation mottle virus
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(89)80190-5

The nucleotide sequence of the genomic RNA of potato leafroll virus was determined and its genetic organization deduced. The RNA is 5882 nucleotides long and contains 6 open reading frames (ORFs) encoding proteins of 70, 70, 56, 28, 23 and 17 kDa. The putative genes for the coat protein (23 kDa) and the RNA-dependent RNA polymerase (70 kDa) were identified by interviral amino acid sequence homologies. For expression of the different ORFS, translational frameshift and readthrough events are proposed.