A role for Myh1 in DNA repair after treatment with strand-breaking and crosslinking chemotherapeutic agents

• Published in: Environmental and molecular mutagenesis Vol. 54; no. 5; pp. 327 - 337
• Main Authors: Jansson, Kristina, Alao, John P., Viktorsson, Kristina, Warringer, Jonas, Lewensohn, Rolf, Sunnerhagen, Per
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.06.2013; Wiley Subscription Services, Inc
• Subjects: 9-1-1 complex; Active Transport, Cell Nucleus - drug effects; Antineoplastic Agents - toxicity; base excision repair; bleomycin; Blotting, Western; Cell and Molecular Biology; Cell Biology; Cell- och molekylärbiologi; Cellbiologi; cisplatin; Cisplatin - toxicity; Cross-Linking Reagents - toxicity; DNA glycosylase; DNA Glycosylases - genetics; DNA Glycosylases - metabolism; DNA Repair - physiology; DNA, Fungal - drug effects; Fluorescent Antibody Technique; Gene Deletion; Medicin och hälsovetenskap; Microbial Viability - drug effects; Microscopy, Fluorescence; mismatch repair; Organoplatinum Compounds - toxicity; Phleomycins - toxicity; Schizosaccharomyces pombe; Schizosaccharomyces pombe Proteins - genetics; Schizosaccharomyces pombe Proteins - metabolism; base excision repair; bleomycin; mismatch repair; DNA glycosylase; 9-1-1 complex; cisplatin
• ISSN: 0893-6692; 1098-2280; 1098-2280
• DOI: 10.1002/em.21784

The highly conserved DNA glycosylase MutY is implicated in repair of oxidative DNA damage, in particular in removing adenines misincorporated opposite 7,8‐dihydro‐8‐oxoguanine (8‐oxo‐G). The MutY homologues (MutYH) physically associate with proteins implicated in replication, DNA repair, and checkpoint signaling, specifically with the DNA damage sensor complex 9‐1‐1 proteins. Here, we ask whether MutYH could have a broader function in sensing and repairing different types of DNA damage induced by conventional chemotherapeutics. Thus, we examined if deletion of the Schizosaccharomyces pombe MutY homologue, Myh1, alone or in combination with deletion of either component of the 9‐1‐1 sensor complex, influences survival after exposure to different classes of DNA damaging chemotherapeutics that do not act primarily by causing 8‐oxoG lesions. We show that Myh1 contributes to survival on genotoxic stresses induced by the oxidizing, DNA double strand break‐inducing, bleomycins, or the DNA crosslinking platinum compounds, particularly in a rad1 mutant background. Exposure of cells to cisplatin leads to a moderate overall accumulation of Myh1 protein. Interestingly, we found that DNA damage induced by phleomycin results in increased chromatin association of Myh1. Further, we demonstrate that Myh1 relocalizes to the nucleus after exposure to hydrogen peroxide or chemotherapeutics, most prominently seen after phleomycin treatment. These observations indicate a wider role of Myh1 in DNA repair and DNA damage‐induced checkpoint activation than previously thought. Environ. Mol. Mutagen. 54:327–337, 2013. © 2013 Wiley Periodicals, Inc.