Understanding the commonalities and differences in genomic organizations across closely related bacteria from an energy perspective

• Published in: Science China. Life sciences Vol. 57; no. 11; pp. 1121 - 1130
• Main Authors: Ma, Qin, Chen, Xin, Liu, Chao, Mao, XiZeng, Zhang, HanYuan, Ji, Fei, Wu, ChunGuo, Xu, Ying
• Format: Journal Article
• Language: English
• Published: Beijing Science China Press 01.11.2014; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Computational Biology - methods; Environment; Escherichia coli; Escherichia coli - genetics; Gene Expression Regulation, Bacterial; Genome, Bacterial; Genomics; Life Sciences; Models, Genetic; Oligonucleotide Array Sequence Analysis; Research Paper; Transcriptional Activation; Transcriptome; 大肠杆菌基因组; 定量模型; 操纵子; 组织; 细菌基因组; 能源; 转录激活; 频率变化; comparative genomics analysis; genomic organization; transcription activation frequency; pathway modeling
• ISSN: 1674-7305; 1869-1889
• DOI: 10.1007/s11427-014-4734-y

The availability of a large number of sequenced bacterial genomes facilitates in-depth studies about why genes （operons） in a bacterial genome are globally organized the way they are. We have previously discovered that （the relative） transcription-activation frequencies among different biological pathways encoded in a genome have a dominating role in the global arrangement of operons. One complicating factor in such a study is that some operons may be involved in multiple pathways with different activation frequencies. A quantitative model has been developed that captures this information, which tends to be minimized by the current global arrangement of operons in a bacterial （and archaeal） genome compared to possible alternative arrangements. A study is carried out here using this model on a collection of 52 closely related Escherichia coli genomes, which revealed interesting new insights about how bacterial genomes evolve to optimally adapt to their environments through adjusting the （relative） genomic locations of the encoding operons of biological pathways once their utilization and hence transcription activation frequencies change, to maintain the above energy-efficiency property. More specifically we observed that it is the frequencies of the transcription activation of pathways relative to those of the other encoded pathways in an organism as well as the variation in the activation frequencies of a specific pathway across the related genomes that play a key role in the observed commonalities and differences in the genomic organizations of genes （and operons） encoding specific pathways across different genomes.