Expression pattern of the human FcRH/IRTA receptors in normal tissue and in B-chronic lymphocytic leukemia
A new family of Ig domain receptors referred to as the immune receptor translocation-associated (IRTA) proteins, FcR homologs (FcRHs) or FcR-like that are expressed in lymphoid cells has been recently described. RNA expression analysis suggests that FcRH1–5/IRTA1–5 are expressed exclusively in subse...
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Published in | International immunology Vol. 18; no. 9; pp. 1363 - 1373 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Oxford University Press
01.09.2006
Oxford Publishing Limited (England) |
Subjects | |
Online Access | Get full text |
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Summary: | A new family of Ig domain receptors referred to as the immune receptor translocation-associated (IRTA) proteins, FcR homologs (FcRHs) or FcR-like that are expressed in lymphoid cells has been recently described. RNA expression analysis suggests that FcRH1–5/IRTA1–5 are expressed exclusively in subsets of the B-cell compartment. We generated mAbs to FcRH1–5/IRTA1–5 and examined their protein expression pattern in normal tissue and in chronic lymphocytic leukemia (CLL) cells. Our data indicated that FcRH1–5/IRTA1–5 were expressed in B-cell sub-populations; however, in some cases, the protein was not expressed in the same B-cell populations as suggested by the RNA expression analysis. FcRH1/IRTA5 was expressed throughout the B-cell lineage starting at the pro-B-cell stage but was down-regulated in plasma cells. FcRH2/IRTA4 was expressed preferentially in memory B cells. FcRH3/IRTA3 was expressed at low levels in naive, germinal center (GC) and memory B cells but was also expressed in NK cells. FcRH4/IRTA1 was expressed in a sub-population of memory B cells associated with mucosal tissue. FcRH5/IRTA2 was expressed in mature B cells and memory B cells and down-regulated in GC cells and, unlike all other B-cell-specific markers, maintained its expression in plasma cells from tonsil, spleen and bone marrow. We examined the expression of FcRH1–5/IRTA1–5 on the surface of CLL cells and found a similar pattern of expression on CLL cells as in the normal mature B cells, except for FcRH3/IRTA3 which was up-regulated in CLL. |
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Bibliography: | ark:/67375/HXZ-WN5TVCSZ-G AbbreviationsAPCallophycoerythrinBM-MNCbone marrow mononuclear cellCLLchronic lymphocytic leukemiaDABdiaminobenzidineECDextracellular domainFcRHFcR homologFFPEformalin fixed paraffin embeddedGCgerminal centerIHCimmunohistochemistryIRTAimmune receptor translocation associatedISHIn situ hybridizationLSMlymphocyte separation mediumMALTmucosa-associated lymphoid tissueMNCmononuclear cellORFopen reading frame Transmitting editor: T. Tedder istex:45DAEE16BAFFC9C2B8ECF4A909F2101EFB7993B9 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0953-8178 1460-2377 |
DOI: | 10.1093/intimm/dxl069 |