Fabrication of gelatin–strontium substituted calcium phosphate scaffolds with unidirectional pores for bone tissue engineering

• Published in: Journal of materials science. Materials in medicine Vol. 26; no. 3; pp. 152 - 12
• Main Authors: Wu, Yu-Chun, Lin, Wei-Yu, Yang, Chyun-Yu, Lee, Tzer-Min
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.03.2015; Springer Nature B.V
• Subjects: Biomaterials; Biomaterials Synthesis and Characterization; Biomedical Engineering and Bioengineering; Biomedical materials; Bone Development; Bones; Calcium; Calcium phosphate; Calcium Phosphates - chemistry; Cell Line; Cell Proliferation; Ceramics; Chemistry and Materials Science; Composites; Compressive strength; Gelatin - chemistry; Gelatins; Glass; Humans; Materials Science; Microscopy, Electron, Scanning; Natural Materials; Polymer Sciences; Porosity; Regenerative Medicine/Tissue Engineering; Scaffolds; Scanning electron microscopy; Skeletal system; Spectroscopy, Fourier Transform Infrared; Strontium; Strontium - chemistry; Studies; Surfaces and Interfaces; Thin Films; Tissue Engineering; Tissue Scaffolds; X-Ray Diffraction; Compressive Strength; Strontium; MG63 Cell; Gelatin; Calcium Phosphate
• ISSN: 0957-4530; 1573-4838; 1573-4838
• DOI: 10.1007/s10856-015-5490-7

This study fabricated homogeneous gelatin–strontium substituted calcium phosphate composites via coprecipitation in a gelatin solution. Unidirectional porous scaffolds with an oriented microtubular structure were then manufactured using freeze–drying technology. The resulting structure and pore alignment were determined using scanning electron microscopy. The pore size were in the range of 200–400 μm, which is considered ideal for the engineering of bone tissue. The scaffolds were further characterized using energy dispersive spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. Hydroxyapatite was the main calcium phosphate compound in the scaffolds, with strontium incorporated into the crystal structure. The porosity of the scaffolds decreased with increasing concentration of calcium-phosphate. The compressive strength in the longitudinal direction was two to threefold higher than that observed in the transverse direction. Our results demonstrate that the composite scaffolds degraded by approximately 20 % after 5 weeks. Additionally, in vitro results reveal that the addition of strontium significantly increased human osteoblastic cells proliferation. Scaffolds containing strontium with a Sr-CaP/(gelatin + Sr-CaP) ratio of 50 % provided the most suitable environment for cell proliferation, particularly under dynamic culture conditions. This study demonstrates the considerable potential of composite scaffolds composed of gelatin–strontium-substituted calcium phosphate for applications in bone tissue engineering.