Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels

• Published in: Scientific reports Vol. 7; no. 1; pp. 8874 - 10
• Main Authors: Chae, Soomin, Lee, Hyun-Kyung, Kim, Yoo-Kyung, Jung Sim, Hyo, Ji, Yoorim, Kim, Chowon, Ismail, Tayaba, Park, Jeen-Woo, Kwon, Oh-Shin, Kang, Beom-Sik, Lee, Dong-Seok, Bae, Jong-Sup, Kim, Sang-Hyun, Min, Kyoung-Jin, Kyu Kwon, Taeg, Park, Mae-Ja, Han, Jin-Kwan, Kwon, Taejoon, Park, Tae-Joo, Lee, Hyun-Shik
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 21.08.2017; Nature Publishing Group UK; Nature Portfolio
• Subjects: Acids; Age; Amino Acid Sequence; Animals; Antioxidants; Cilia; Conserved Sequence; Cysteine; Embryogenesis; Enzymes; Epistasis; Gene Expression Regulation, Developmental; Gene Knockdown Techniques; Hybridization; Hydrogen peroxide; Life sciences; Organogenesis - genetics; Peroxiredoxin; Peroxiredoxins - chemistry; Peroxiredoxins - genetics; Peroxiredoxins - metabolism; Phenotype; Pronephros - embryology; Pronephros - metabolism; Proteins; Reactive oxygen species; Reactive Oxygen Species - metabolism; Retinoic acid; Tretinoin - metabolism; Wnt protein; Wnt Signaling Pathway; Xenopus laevis
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-09262-6

Peroxiredoxin1 (Prdx1) is an antioxidant enzyme belonging to the peroxiredoxin family of proteins. Prdx1 catalyzes the reduction of H O and alkyl hydroperoxide and plays an important role in different biological processes. Prdx1 also participates in various age-related diseases and cancers. In this study, we investigated the role of Prdx1 in pronephros development during embryogenesis. Prdx1 knockdown markedly inhibited proximal tubule formation in the pronephros and significantly increased the cellular levels of reactive oxygen species (ROS), which impaired primary cilia formation. Additionally, treatment with ROS (H O ) severely disrupted proximal tubule formation, whereas Prdx1 overexpression reversed the ROS-mediated inhibition in proximal tubule formation. Epistatic analysis revealed that Prdx1 has a crucial role in retinoic acid and Wnt signaling pathways during pronephrogenesis. In conclusion, Prdx1 facilitates proximal tubule formation during pronephrogenesis by regulating ROS levels.