HPLC-DAD optimization of quantification of vescalagin, gallic and ellagic acid in chestnut tannins

• Published in: Heliyon Vol. 9; no. 8; p. e18993
• Main Authors: Richard-Dazeur, Charline, Jacolot, Philippe, Niquet-Léridon, Céline, Goethals, Luc, Barbezier, Nicolas, Anton, Pauline M.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.08.2023; Elsevier
• Subjects: Chestnut tannins; Ellagic acid; Gallic acid; HPLC-DAD; Life Sciences; Polyphenol; Vescalagin; Polyphenol; HPLC-DAD; Gallic acid; Vescalagin; Ellagic acid; Chestnut tannins; HPLC-DAD Polyphenol Gallic acid Vescalagin Ellagic acid Chestnut tannins
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2023.e18993

The quantification of hydrolysable polyphenols such as gallic, ellagic acid and vescalagin by HPLC-DAD is classically run after methanol extraction as a reference solvent. Water extraction is usually discarded because of a lower obtention of total polyphenol content compared to methanol extraction. In our study, methanol was compared to water extraction in both the total polyphenol content method and the HPLC-DAD analysis. Total polyphenol content in water extraction was lower than in methanol extraction, but water extraction gave better results on HPLC-DAD. In conclusion, total polyphenol content cannot be used as reference to choose the solvent of extraction to quantify some polyphenols by HPLC-DAD because of the specific properties of each polyphenol. Indeed, recovery results obtained on hydrolysable polyphenols with water extraction were better and with a lower variability than following methanol extraction. •Quantification of gallic acid, ellagic acid and vescalagin by HPLC-DAD.•Appropriate extraction method for quantification of hydrolysable polyphenol.•Validation of water extraction to quantify hydrolysable polyphenol.