Integrated Transcriptome and Metabolome Analysis of Rice Leaves Response to High Saline-Alkali Stress

• Published in: International journal of molecular sciences Vol. 24; no. 4; p. 4062
• Main Authors: Qian, Guangtao, Wang, Mingyu, Wang, Xiaoting, Liu, Kai, Li, Ying, Bu, Yuanyuan, Li, Lixin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.02.2023; MDPI
• Subjects: ABC transporter; Abiotic stress; Agricultural production; Amino acids; Analysis; Antioxidants; Biosynthesis; China; Dams; Enzymes; Gene expression; Genes; Germplasm; glutathione metabolism; Homeostasis; Linoleic acid; linoleic acid metabolism; Lipid metabolism; Lipid peroxidation; Lipids; Metabolism; Metabolites; Molecular modelling; Oryza sativa; Osmosis; Physiological aspects; Plant breeding; Plant growth; Polyamines; Rice; saline–alkali stress; Salt; Signal transduction; TCA cycle; Transcriptomes; transcriptomics and metabolomics; Tricarboxylic acid cycle; Unsaturated fatty acids; China; glutathione metabolism; linoleic acid metabolism; rice; transcriptomics and metabolomics; TCA cycle; saline–alkali stress
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24044062

Rice ( ) is one of the most important crops grown worldwide, and saline-alkali stress seriously affects the yield and quality of rice. It is imperative to elucidate the molecular mechanisms underlying rice response to saline-alkali stress. In this study, we conducted an integrated analysis of the transcriptome and metabolome to elucidate the effects of long-term saline-alkali stress on rice. High saline-alkali stress (pH > 9.5) induced significant changes in gene expression and metabolites, including 9347 differentially expressed genes (DEGs) and 693 differentially accumulated metabolites (DAMs). Among the DAMs, lipids and amino acids accumulation were greatly enhanced. The pathways of the ABC transporter, amino acid biosynthesis and metabolism, glyoxylate and dicarboxylate metabolism, glutathione metabolism, TCA cycle, and linoleic acid metabolism, etc., were significantly enriched with DEGs and DAMs. These results suggest that the metabolites and pathways play important roles in rice's response to high saline-alkali stress. Our study deepens the understanding of mechanisms response to saline-alkali stress and provides references for molecular design breeding of saline-alkali resistant rice.