Structural, Surface, in vitro Bacterial Adhesion and Biofilm Formation Analysis of Three Dental Restorative Composites

• Published in: Materials Vol. 8; no. 6; pp. 3221 - 3237
• Main Authors: Azam, Maria T, Khan, Abdul S, Muzzafar, Danish, Faryal, Rani, Siddiqi, Saadat A, Ahmad, Riaz, Chauhdry, Aqif A, Rehman, Ihtesham U
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 03.06.2015; MDPI
• Subjects: Adhesion; Bacteria; bacterial adhesion; Biofilms; Chemical composition; Composite materials; Contact angle; Dental cement; dental composites; Dental materials; Dental restorative materials; Dentistry; Escherichia; Fourier transforms; Information technology; Intervals; Particle size; Polymerization; Pseudomonas aeruginosa; Restoration; spectroscopic analysis; Staphylococcus aureus; surface properties; Wettability; Lahore Pakistan; Pakistan
• ISSN: 1996-1944; 1996-1944
• DOI: 10.3390/ma8063221

This study was conducted to investigate the relationship between dental materials and bacterial adhesion on the grounds of their chemical composition and physical properties. Three commercially available dental restorative materials (Filtek(TM)Z350, Filtek(TM)P90 and Spectrum registered TPH registered ) were structurally analyzed and their wettability and surface roughness were evaluated by using Fourier Transform Infrared Spectroscopy, Contact Angle Measurement and Atomic Force Microscopy, respectively. These materials were molded into discs and tested with three bacterial strains (Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia) for microbial attachment. The bacterial adhesion was observed at different time intervals, i.e., 0 h, 8 h, 24 h, 48 h and 72 h, along with Colony Forming Unit Count and Optical Density measurement of the media. It was found that all materials showed a degree of conversion with time intervals, i.e., 0 h, 8 h, 24 h, 48 h and 72 h, which led to the availability of functional groups (N-H and C-H) that might promote adhesion. The trend in difference in the extent of bacterial adhesion can be related to particle size, chemical composition and surface wettability of the dental materials.