Immunogenicity and tolerance following HIV-1/HBV plant-based oral vaccine administration

• Published in: Vaccine Vol. 26; no. 35; pp. 4477 - 4485
• Main Authors: Guetard, Denise, Greco, Raffaella, Cervantes Gonzalez, Minerva, Celli, Susanna, Kostrzak, Anna, Langlade-Demoyen, Pierre, Sala, Francesco, Wain-Hobson, Simon, Sala, Monica
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 18.08.2008; Elsevier; Elsevier Limited
• Subjects: Administration, Oral; AIDS Vaccines; AIDS Vaccines - administration & dosage; AIDS Vaccines - adverse effects; AIDS Vaccines - immunology; Allergy and Immunology; Animals; Applied microbiology; Biological and medical sciences; CD8-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes - immunology; Epitopes; Epitopes - genetics; Epitopes - immunology; Female; Flow Cytometry; Flowers & plants; Fundamental and applied biological sciences. Psychology; HBV; Hepatitis B Vaccines; Hepatitis B Vaccines - administration & dosage; Hepatitis B Vaccines - adverse effects; Hepatitis B Vaccines - immunology; Hepatitis B virus; Hepatitis B virus - genetics; HIV; HIV-1; HIV-1 - genetics; Human immunodeficiency virus; Human immunodeficiency virus 1; Immune system; Immunization, Secondary; Immunization, Secondary - methods; Immunology; Infections; Life Sciences; Lymph Nodes; Lymph Nodes - immunology; Lymphocyte Activation; Lymphocyte Subsets; Lymphocyte Subsets - immunology; Lymphocytes; Metabolites; Mice; Microbiology; Miscellaneous; Nicotiana; Plants, Genetically Modified; Proteins; Rodents; T-Lymphocytes, Regulatory; T-Lymphocytes, Regulatory - immunology; Tobacco; Tolerance; Transgenic plants; Vaccine; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, DNA; Vaccines, DNA - immunology; Vaccines, Edible; Vaccines, Edible - administration & dosage; Vaccines, Edible - adverse effects; Vaccines, Edible - immunology; Vaccines, Virosome; Vaccines, Virosome - administration & dosage; Vaccines, Virosome - adverse effects; Vaccines, Virosome - immunology; Vaccinology; Viral infections; Viral Proteins; Viral Proteins - biosynthesis; Viral Proteins - genetics; Virology; VLPs; HIV-1; Tolerance; VLPs; Vaccine; HBV; Transgenic plants; Virus; Immunogenicity; HIV-1 virus; Hepadnaviridae; Oral administration; Orthohepadnavirus; Retroviridae; HIV-1;HBV;Transgenic plants;VLPs;Vaccine;Tolerance; Human immunodeficiency virus; Hepatitis B virus; Lentivirus
• ISSN: 0264-410X; 1873-2518; 0264-410X
• DOI: 10.1016/j.vaccine.2008.06.059

Abstract Transgenic tobacco plants expressing a HIV-1 polyepitope associated with hepatitis B (HBV) virus-like particles (VLPs) were previously described. It is demonstrated here that oral administration of these transgenic plants to humanized HSB mice to boost DNA-priming can elicit anti-HIV-1 specific CD8+ T cell activation detectable in mesenteric lymph nodes. Nevertheless, a significant regulatory T cell activation was induced in vivo by the vaccination protocols. The balance between tolerance and immunogenicity remains the main concern in the proof of concept of plant-based vaccine.