Structure of Sediment-Associated Microbial Communities along a Heavy-Metal Contamination Gradient in the Marine Environment

• Published in: Applied and Environmental Microbiology Vol. 71; no. 2; pp. 679 - 690
• Main Authors: Gillan, David C, Danis, Bruno, Pernet, Philippe, Joly, Guillemette, Dubois, Philippe
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.02.2005
• Subjects: Animal, plant and microbial ecology; Applied ecology; Biological and medical sciences; Colony Count, Microbial; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Ecosystem; Ecotoxicology, biological effects of pollution; Electrophoresis, Agar Gel - methods; Fundamental and applied biological sciences. Psychology; Geologic Sediments - chemistry; Geologic Sediments - microbiology; Gram-Negative Bacteria - classification; Gram-Negative Bacteria - genetics; Gram-Negative Bacteria - isolation & purification; Heavy metal content; In Situ Hybridization, Fluorescence; Indoles; Marine and brackish environment; Marine ecology; Metals, Heavy - analysis; Microbial Ecology; Microbiology; Molecular Sequence Data; Phylogeny; Polychlorinated Biphenyls - analysis; RNA, Ribosomal, 16S - genetics; Seawater - chemistry; Seawater - microbiology; Sequence Analysis, DNA; Water Pollutants, Chemical - analysis; Water pollution; Marine environment; Gradient; Sediments; Microbial community; Contamination; Heavy metal
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.71.2.679-690.2005

Microbial community composition and structure were characterized in marine sediments contaminated for >80 years with cadmium, copper, lead, and zinc. Four sampling sites that encompass a wide range of sediment metal loads were compared in a Norwegian fjord (Sørfjord). HCl-extractable metals and organic matter constantly decreased from the most contaminated site (S1) to the control site (S4). All sampling sites presented low polychlorinated biphenyl (PCB) concentrations ([Sigma]₇PCB < 7.0 ng g [dry weight]⁻¹). The biomass ranged from 4.3 x 10⁸ to 13.4 x 10⁸ cells g (dry weight) of sediments⁻¹ and was not correlated to metal levels. Denaturing gradient gel electrophoresis indicated that diversity was not affected by the contamination. The majority of the partial 16S rRNA sequences obtained were classified in the [gamma]- and [delta]-Proteobacteria and in the Cytophaga-Flexibacter-Bacteroides (CFB) bacteria. Some sequences were closely related to other sequences from polluted marine sediments. The abundances of seven phylogenetic groups were determined by using fluorescent in situ hybridization (FISH). FISH was impaired in S1 by high levels of autofluorescing particles. For S2 to S4, the results indicated that the HCl-extractable Cu, Pb, and Zn were negatively correlated with the abundance of [gamma]-Proteobacteria and CFB bacteria. [delta]-Proteobacteria were not correlated with HCl-extractable metals. Bacteria of the Desulfosarcina-Desulfococcus group were detected in every site and represented 6 to 14% of the DAPI (4',6'-diamidino-2-phenylindole) counts. Although factors other than metals may explain the distribution observed, the information presented here may be useful in predicting long-term effects of heavy-metal contamination in the marine environment.