Investigation of Chromosome 1 Aberrations in the Lymphocytes of Prostate Cancer and Benign Prostatic Hyperplasia Patients by Fluorescence in situ Hybridization

• Published in: Cancer management and research Vol. 13; pp. 4291 - 4298
• Main Authors: Miszczyk, Justyna, Przydacz, Mikołaj, Zembrzuski, Michał, Chłosta, Piotr L
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2021; Taylor & Francis Ltd; Dove; Dove Medical Press
• Subjects: benign prostatic hyperplasia; Biomarkers; Cancer; Cancer patients; Cancer therapies; Care and treatment; Cell cycle; chromosome 1; Chromosomes; fish method; Fluorescence; Genetic aspects; Genetic research; Genital diseases; Health aspects; Hybridization; Hyperplasia; Hypertrophy; Lymphocytes; Medical research; Medicine, Experimental; Original Research; Prostate; Prostate cancer; Radiation; Radiation therapy; Radiotherapy; radiotherapy planning; X-rays; Poland; radiotherapy planning; FISH method; prostate cancer; chromosome 1; biomarkers; benign prostatic hyperplasia
• ISSN: 1179-1322; 1179-1322
• DOI: 10.2147/CMAR.S293249

Radiotherapy is one of the most common treatments for prostate cancer. Finding a useful predictor of the therapeutic outcome is crucial as it increases the efficacy of treatment planning. This study investigated the individual susceptibility to radiation based on chromosome 1 aberration frequency measured by the FISH (fluorescence in situ hybridization) method. Whole blood samples were collected from 27 prostate cancer (PCa) patients and 32 subjects with benign prostatic hyperplasia (BPH), who were considered as a control group. Samples were irradiated with 2 Gy of x-rays, cultured, harvested, and used in the FISH procedure. After irradiation, significantly higher levels of all studied chromosome 1 aberrations (except for deletions) in the group of PCa patients were revealed. Furthermore, in the lymphocytes of cancer patients, nearly five-fold higher frequencies of acentric fragments were observed compared to the BPH group. The highest individual radiosensitivities for all estimated biomarkers were seen in PCa patient cells who reported cancer incidence in the immediate family (CIF+). The differences in chromosome 1 aberrations between PCa and BPH demonstrate that lymphocytes taken from patients with prostate cancer have higher radiosensitivity which might be related to hereditary or familiar inclinations. Therefore, this technique may find future application in searching biomarkers of the cellular radiotherapy response in prostate cancer patients.