Surface Plasmon Resonance Microscopy Based on Total Internal Reflection

• Published in: Biosensors (Basel) Vol. 13; no. 2; p. 261
• Main Authors: Zhang, Teliang, Wang, Xueliang, Zeng, Youjun, Huang, Songfeng, Dai, Xiaoqi, Kong, Weifu, Liu, Qian, Chen, Jiajie, Qu, Junle, Shao, Yonghong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.02.2023; MDPI
• Subjects: Algorithms; Analysis; Antigens; biomolecular interaction; Electric fields; Fourier transforms; Glass substrates; Gold; IgG antibody; Immunoglobulin G; Lasers; Light; Medical research; Methods; Microscopy; Nanoparticles; Resonance; Spatial discrimination; Spatial resolution; Surface plasmon resonance; surface plasmon resonance microscopy; Surface plasmon resonance spectroscopy; total internal reflection; China; surface plasmon resonance microscopy; biomolecular interaction; surface plasmon resonance; total internal reflection
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios13020261

Surface plasmon resonance microscopy (SPRM) has been widely employed in biological fields because of its high spatial resolution and label-free detection modality. In this study, SPRM based on total internal reflection (TIR) is studied via a home-built SPRM system, and the principle of imaging of a single nanoparticle is analyzed as well. By designing a ring filter and combining it with the deconvolution algorithm in Fourier space, the parabolic tail of the nanoparticle image is removed, in which a spatial resolution of 248 nm is obtained. In addition, we also measured the specific binding between the human IgG antigen and goat anti-human IgG antibody using the TIR-based SPRM. The experimental results have proved that the system can image sparse nanoparticles and monitor biomolecular interactions.