LygA retention on the surface of Listeria monocytogenes via its interaction with wall teichoic acid modulates bacterial homeostasis and virulence

• Published in: PLoS pathogens Vol. 19; no. 6; p. e1011482
• Main Authors: Yao, Hao, Li, Guo, Xiong, Xianglian, Jin, Fanxin, Li, Sirui, Xie, Xinyu, Zhong, Dan, Zhang, Renling, Meng, Fanzeng, Yin, Yuelan, Jiao, Xin'an
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 28.06.2023
• Subjects: Analysis; Bacteria; Binding; Biology and Life Sciences; Biosynthesis; Cell cycle; Cell division; Cell surface; Cell walls; Control; Glycine; Glycopolymers; Glycosylation; Gram-positive bacteria; Health aspects; Homeostasis; Identification and classification; Listeria; Listeria monocytogenes; Listeriosis; Medicine and Health Sciences; Metabolism; Metabolites; Pathogenesis; Physical Sciences; Polyclonal antibodies; Prevention; Proteins; Retention; Risk factors; Signal transduction; Tryptophan; Virulence; Virulence (Microbiology); Virulence factors; China
• ISSN: 1553-7374; 1553-7366; 1553-7374
• DOI: 10.1371/journal.ppat.1011482

Wall teichoic acid (WTA) is the abundant cell wall-associated glycopolymer in Gram-positive bacteria, playing crucial roles in surface proteins retention, bacterial homeostasis, and virulence. The WTA glycosylation of Listeria monocytogenes is essential for surface anchoring of virulence factors, whereas the nature and function of the noncovalent interactions between cell wall-associated proteins and WTA are less unknown. In this study, we found that galactosylated WTA (Gal-WTA) of serovar (SV) 4h L. monocytogenes plays a key role in modulating the novel glycine-tryptophan (GW) domain-containing autolysin protein LygA through direct interactions. Gal-deficient WTA of Lm XYSN (ΔgalT) showed a dramatic reduction of LygA on the cell surface. We demonstrated that LygA binds to Gal-WTA through the GW domains, and the binding affinity is associated with the number of GW motifs. Moreover, we confirmed the direct Gal-dependent binding of the GW protein Auto from the type I WTA strain, which has no interaction with rhamnosylated WTA, indicating that the complexity of both WTA and GW proteins affect the coordination patterns. Importantly, we revealed the crucial roles of LygA in facilitating bacterial homeostasis as well as crossing the intestinal and blood-brain barriers. Altogether, our findings suggest that both the glycosylation patterns of WTA and a fixed numbers of GW domains are closely associated with the retention of LygA on the cell surface, which promotes the pathogenesis of L. monocytogenes within the host.