Prevalence and persistence of potentially pathogenic and antibiotic resistant bacteria during anaerobic digestion treatment of cattle manure

• Published in: Bioresource technology Vol. 153; pp. 284 - 291
• Main Authors: Resende, Juliana Alves, Silva, Vânia Lúcia, de Oliveira, Tamara Lopes Rocha, de Oliveira Fortunato, Samuel, da Costa Carneiro, Jailton, Otenio, Marcelo Henrique, Diniz, Cláudio Galuppo
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.02.2014; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Anaerobic digestion; Anaerobiosis - drug effects; Animal productions; Animals; Anti-Bacterial Agents - pharmacology; Antimicrobial resistance; Bacteria - growth & development; Bacteria - isolation & purification; Biodegradation, Environmental - drug effects; Biofertilizer dairy cattle manure; Biofuel production; Biofuels - microbiology; Biogas; Biological and medical sciences; Biological treatment of sewage sludges and wastes; Bioreactors - microbiology; Biotechnology; Cattle; Colony Count, Microbial; Drug Resistance, Microbial; Energy; Environment and pollution; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Humans; Industrial applications and implications. Economical aspects; Manure - microbiology; Microbial Sensitivity Tests; Pathogenic bacteria; Pilot Projects; Refuse Disposal - methods; Species Specificity; Terrestrial animal productions; Use of agricultural and forest wastes. Biomass use, bioconversion; Vertebrates; Biogas; Biofertilizer dairy cattle manure; Antimicrobial resistance; Pathogenic bacteria; Anaerobic digestion; Farming animal; Prevalence; Dairy cattle; Antimicrobial agent; Resistance; Biological fertilizer; Vertebrata; Antibiotic; Mammalia; Pathogenic; Bacteria; Artiodactyla; Cattle manure; Ungulata
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.12.007

•Sustainable handling of anaerobic digestion and their effluents are needed.•Effluents from biodigesters are widely spread into land used for the agriculture.•Putative pathogenic bacteria persist after anaerobic digestion of cattle manure.•Antimicrobial-resistant bacteria are prevalent in the effluents from biodigesters.•Medically important bacteria imposes sanitary risks to the anaerobic digestion. Anaerobic digestion figures as a sustainable alternative to avoid discharge of cattle manure in the environment, which results in biogas and biofertilizer. Persistence of potentially pathogenic and drug-resistant bacteria during anaerobic digestion of cattle manure was evaluated. Selective cultures were performed for enterobacteria (ENT), non-fermenting Gram-negative rods (NFR) and Gram-positive cocci (GPC). Antimicrobial susceptibility patterns were determined and a decay of all bacterial groups was observed after 60days. Multidrug-resistant bacteria were detected both the influent and effluent. GPC, the most prevalent group was highly resistant against penicillin and levofloxacin, whereas resistance to ampicillin, ampicillin-sulbactam and chloramphenicol was frequently observed in the ENT and NFR groups. The data point out the need of discussions to better address management of biodigesters and the implementation of sanitary and microbiological safe treatments of animal manures to avoid consequences to human, animal and environmental health.