Effects of alginate oligomer on the expression of cell cycle- and stress-related genes in Chlamydomonas reinhardtii

• Published in: Bioscience, biotechnology, and biochemistry Vol. 81; no. 6; pp. 1254 - 1260
• Main Authors: Ueno, Mikinori, Nishiguchi, Tomoki, Takeshita, Satoshi, Yamaguchi, Kenichi, Oda, Tatsuya
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 03.06.2017; Oxford University Press
• Subjects: Algal Proteins - genetics; Algal Proteins - metabolism; alginate; alginate oligomer; Alginates - pharmacology; Antioxidants - pharmacology; Ascorbate Peroxidases - genetics; Ascorbate Peroxidases - metabolism; Cell Cycle - drug effects; Cell Cycle - genetics; Chlamydomonas reinhardtii; Chlamydomonas reinhardtii - drug effects; Chlamydomonas reinhardtii - genetics; Chlamydomonas reinhardtii - growth & development; Chlamydomonas reinhardtii - metabolism; Copper Sulfate - antagonists & inhibitors; Copper Sulfate - toxicity; CuSO; Cyclin A - genetics; Cyclin A - metabolism; Cyclin B - genetics; Cyclin B - metabolism; Cyclin D - genetics; Cyclin D - metabolism; Cytotoxins - antagonists & inhibitors; Cytotoxins - toxicity; gene expression; Gene Expression Regulation - drug effects; Glucuronic Acid - pharmacology; Hexuronic Acids - pharmacology; Polymerization; Reactive Oxygen Species - antagonists & inhibitors; Reactive Oxygen Species - metabolism; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; alginate; CuSO; gene expression; alginate oligomer; CuSO4; Chlamydomonas reinhardtii
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2017.1292836

Enzymatically prepared alginate oligomer (AO) promoted the growth of Chlamydomonas reinhardtii in a concentration-dependent manner. AO at 2.5 mg/mL induced increase in expression levels of cyclin A, cyclin B, and cyclin D in C. reinhardtii. CuSO 4 at 100 μM suppressed the growth of C. reinhardtiin, and AO at 2.5 mg/mL significantly alleviated the toxicity of CuSO 4 . Increased intracellular reactive oxygen species level in C. reinhardtii induced by CuSO 4 was reduced by AO. After cultivation with CuSO 4 at 100 μM, expression levels of ascorbate peroxidase and superoxide dismutase in C. reinhardtii were increased, and AO reduced the increased levels of these enzymes. These results suggest that AO exhibits beneficial effects on C. reinhardtii through influencing the expression of various genes not only at normal growth condition but also under CuSO 4 stress.