CHARACTERISTICS OF MURAYAMA VIRUS IN VARIOUS CELL CULTURES AND LABORATORY ANIMALS

Some biological properties of Murayama virus, a new paramyxovirus, were studied. The virus grew well in primary monkey kidney cells as well as embryonated eggs, while the virus yields in primary chick embryo and BHK-21 cells were much lower. The infected BHK-21 cells formed large syncytia and showed...

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Published inJapanese Journal of Medical Science and Biology Vol. 34; no. 1; pp. 9 - 20
Main Authors NISHIKAWA, FUMIO, YAMAMOTO, KIICHI, SUGIYAMA, TEIJI
Format Journal Article
LanguageEnglish
Published Japan National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee 01.01.1981
Subjects
Animals
Animals, Laboratory - microbiology
Birds
Cell Fusion
Cells, Cultured
Chick Embryo
Chickens
Cricetinae
Freezing
Guinea Pigs
Haplorhini
Kidney - microbiology
Mice
Paramyxoviridae - growth & development
Paramyxoviridae - pathogenicity
Trypsin - pharmacology
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Summary:Some biological properties of Murayama virus, a new paramyxovirus, were studied. The virus grew well in primary monkey kidney cells as well as embryonated eggs, while the virus yields in primary chick embryo and BHK-21 cells were much lower. The infected BHK-21 cells formed large syncytia and showed typical hemadsorption, but did not produce any detectable amount of hemagglutinin in the culture fluid. The virus yields were very low in Vero, LLC-MK2 and MDCK cells at first passages. The addition of trypsin to the medium enhanced virus growth in Vero and LLC-MK2 but not in MDCK cells. Cell fusion activity of the virus was observed in Molt-4 cells. Hemolytic activity was enhanced by freeze-thawing. Several species of mammals and birds were susceptible to experimental infections with the virus as evidenced by seroconversion and positive virus isolation without showing any clinical signs.
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ISSN:0021-5112
1884-2828
DOI:10.7883/yoken1952.34.9