CHARACTERISTICS OF MURAYAMA VIRUS IN VARIOUS CELL CULTURES AND LABORATORY ANIMALS
Some biological properties of Murayama virus, a new paramyxovirus, were studied. The virus grew well in primary monkey kidney cells as well as embryonated eggs, while the virus yields in primary chick embryo and BHK-21 cells were much lower. The infected BHK-21 cells formed large syncytia and showed...
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Published in | Japanese Journal of Medical Science and Biology Vol. 34; no. 1; pp. 9 - 20 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Japan
National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
01.01.1981
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Subjects | |
Online Access | Get full text |
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Summary: | Some biological properties of Murayama virus, a new paramyxovirus, were studied. The virus grew well in primary monkey kidney cells as well as embryonated eggs, while the virus yields in primary chick embryo and BHK-21 cells were much lower. The infected BHK-21 cells formed large syncytia and showed typical hemadsorption, but did not produce any detectable amount of hemagglutinin in the culture fluid. The virus yields were very low in Vero, LLC-MK2 and MDCK cells at first passages. The addition of trypsin to the medium enhanced virus growth in Vero and LLC-MK2 but not in MDCK cells. Cell fusion activity of the virus was observed in Molt-4 cells. Hemolytic activity was enhanced by freeze-thawing. Several species of mammals and birds were susceptible to experimental infections with the virus as evidenced by seroconversion and positive virus isolation without showing any clinical signs. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0021-5112 1884-2828 |
DOI: | 10.7883/yoken1952.34.9 |