Occurrence of free ceramides in Bacteroides fragilis NCTC 9343

The occurrence of free ceramides at high concentrations was demonstrated in the chloroform-methanol extractable lipids of Bacteroides fragilis NCTC 9343. The long-chain bases were isolated from the free ceramides and identified as branched and normal saturated dihydroxy bases with carbon chains cons...

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Bibliographic Details
Published inJournal of biochemistry (Tokyo) Vol. 86; no. 2; pp. 311 - 320
Main Authors MIYAGAWA, Eiichi, AZUMA, Ryozo, SUTO, Tsuneji, YANO, Ikuya
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.08.1979
Subjects
Bacteroides fragilis - analysis
Ceramides - analysis
Chromatography, Gas
Chromatography, Thin Layer
Fatty Acids - analysis
Mass Spectrometry
Osmolar Concentration
Spectrophotometry, Infrared
Sphingolipids - analysis
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Summary:The occurrence of free ceramides at high concentrations was demonstrated in the chloroform-methanol extractable lipids of Bacteroides fragilis NCTC 9343. The long-chain bases were isolated from the free ceramides and identified as branched and normal saturated dihydroxy bases with carbon chains consisting of 17, 18, and 19 atoms. The major fatty acid was 3-hydroxy 15-methylhexadecanoic acid. The major molecular species of the ceramides were identified by gas chromatography-mass spectometry and gas chromatography of the cleaved products as LCB-d-iso17: 0-3-OH iso17: 0 FA, LCB-d-anteiso17: 0-3-OH iso17: 0 FA, LCB-d-iso18: 0-3-OH iso17: 0 FA, and LCB-d-anteisol9: 0-3-OH iso17: 0 FA.
Bibliography:F60
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ArticleID:86.2.311
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content type line 23
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a132528