Characteristics of an Extended Gate Field-Effect Transistor for Glucose Sensing Using an Enzyme-Containing Silk Fibroin Membrane as the Bio-Chemical Component

• Published in: Biosensors (Basel) Vol. 10; no. 6; p. 57
• Main Authors: Koike, Kazuto, Sasaki, Taihou, Hiraki, Kenta, Ike, Kodai, Hirofuji, Yuichi, Yano, Mitsuaki
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.05.2020; MDPI
• Subjects: Aqueous electrolytes; Biosensing Techniques; biosensor; Biosensors; Chemical composition; Chemoreception; Detection; Diabetes; EGFET; Electrodes; Electrolytes; Endurance; Enzymes; Fibroins - chemistry; Fibroins - metabolism; Field effect transistors; Fourier transforms; Glass substrates; Glucose; Glucose - analysis; Glucose - metabolism; Glucose oxidase; Glucose Oxidase - chemistry; Glucose Oxidase - metabolism; Graphene; Heat tolerance; immobilization; Membranes; Researchers; Retention time; Room temperature; Saliva; Saliva - chemistry; Semiconductor devices; Sensors; Silk; Silk - chemistry; Silk - metabolism; Silk fibroin; Surface Properties; Sweat - chemistry; Temperature tolerance; Titanium - chemistry; Titanium dioxide; Transistors, Electronic; Japan; biosensor; immobilization; EGFET; glucose oxidase; silk fibroin
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios10060057

The characteristics of a glucose sensor based on an ion-sensitive TiO /Ti extended gate electrode field-effect transistor (EGFET) are reported. A glucose oxidase-containing silk fibroin membrane was immobilized on a TiO /Ti surface as the bio-sensing component. This EGFET-type biosensor was estimated to be able to detect a glucose concentration as low as 0.001 mg/mL in an aqueous electrolyte, which enables the sensing of glucose in the saliva and sweat. The endurance of this sensor was also examined, and it was found that the retention time of the original sensitivity for repeated use at room temperature was more than 30 days, with a high heat tolerance temperature close to 60 °C.