Hydrophobic features of EPS extracted from anaerobic granular sludge: an investigation based on DAX-8 resin fractionation and size exclusion chromatography

• Published in: Applied microbiology and biotechnology Vol. 101; no. 8; pp. 3427 - 3438
• Main Authors: Cao, Feishu, Bourven, Isabelle, Lens, Piet N.L., van Hullebusch, Eric D., Pechaud, Yoan, Guibaud, Gilles
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.04.2017; Springer; Springer Nature B.V; Springer Verlag
• Subjects: Anaerobiosis; Analysis; Biochemical composition; Biofilms; Biomedical and Life Sciences; Biotechnology; Carbon; Chemical and Process Engineering; Chemical Fractionation; Chromatography; Chromatography, Gel; Contact angle; Engineering Sciences; Environmental Biotechnology; Ethanol; Formaldehyde; Fractionation; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Investigations; Life Sciences; Microbial Genetics and Genomics; Microbiology; Molecular Weight; Organic carbon; Polymers; Polymers - chemistry; Polymers - isolation & purification; Proteins; Qualitative analysis; Reagents; Sewage - chemistry; Sludge; Studies; Sulfates; Surface active agents; United States > US; EPS extraction; Hydrophobicity; SEC/absorbance 210 nm; DAX-8 resin; Anaerobic granular sludge; SEC/absorbance 210 nm; DAX-8 resin Hydrophobicity
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-016-8053-z

The hydrophobic fractionation of extracellular polymeric substances (EPS) extracted from anaerobic granular sludge was performed on the DAX-8 resin (two elution pH conditions, i.e., pH 2 and pH 5 were tested). The impact of seven different EPS extraction methods on EPS hydrophobicity features was assessed. The results showed that the extraction methods and bulk solution pH influenced dramatically the biochemical composition of the EPS, and in turn, the hydrophobicity determined. Besides, EPS extracting reagents i.e., formaldehyde, ethanol, sodium dodecyl sulfate (SDS), and Tween 20 not only introduced extra carbon content in the total organic carbon (TOC) measurement but also interacted with the DAX-8 resin. By comparing the apparent molecular weight (aMW) distribution of untreated and pH-adjusted EPS samples, more complete EPS aMW information was preserved at pH 5. Thus, elution at pH 5 was preferred in this study for the qualitative analysis of EPS hydrophobic features. The hydrophobic fraction of EPS retained by the resin at pH 5 was ascribed to a wide aMW range, ranging from >440 to 0.3 kDa. Within this range, EPS molecules ranging from 175 to 31 kDa were mostly retained by the DAX-8 resin, which indicates that these EPS molecules are highly hydrophobic.