Downregulations of AKT/mTOR Signaling Pathway for Salmonella -Mediated Suppression of Matrix Metalloproteinases-9 Expression in Mouse Tumor Models

• Published in: International journal of molecular sciences Vol. 19; no. 6; p. 1630
• Main Authors: Tsao, Yu-Tzu, Kuo, Chun-Yu, Cheng, Shun-Ping, Lee, Che-Hsin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 31.05.2018; MDPI
• Subjects: AKT protein; Animal models; Animals; Cell adhesion & migration; Cell Line, Tumor; Cell Movement; Disease Models, Animal; Gelatinase B; Gene Expression Regulation; Humans; Kinases; Lung nodules; Lungs; Matrix Metalloproteinase 9 - genetics; Matrix metalloproteinases; Metastases; Metastasis; Mice; Neoplasm Metastasis; Neoplasms - genetics; Neoplasms - metabolism; Neoplasms - pathology; Nodules; Phosphorylation; Proteins; Proto-Oncogene Proteins c-akt - metabolism; Rapamycin; Salmonella; Salmonella - physiology; Signal Transduction; TOR protein; TOR Serine-Threonine Kinases - metabolism; tumor-targeting; Tumors; Wound healing; Xenograft Model Antitumor Assays; Salmonella; metastasis; matrix metalloproteinases; tumor-targeting
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms19061630

The roles of Matrix MetalloProteinases (MMPs), such as MMP-9, in tumor metastasis are well studied, and this in turns stimulates the development of MMP inhibitors as antitumor agents. Previously, accumulation was observed in the metastatic nodules of the lungs after systemic administration. significantly enhanced the survival of the pulmonary metastatic tumor-bearing mice. Based on our previous observation, we hypothesized that could affect metastasis-related protein expression. The treatment of clearly reduced the expression of MMP-9. Meanwhile, the MMP-9 related signaling pathways, including Phosph-Protein Kinase B (P-AKT) and Phosph-mammalian Targets Of Rapamycin (P-mTOR) were decreased after a treatment. The inhibited tumor cell migration by wound-healing and Transwell assay. The anti-metastatic effects of were evaluated in mice bearing experimental metastasis tumor models. Consequently, inhibited the expression of MMP-9 by reducing the AKT/mTOR pathway and metastatic nodules in vivo.