Osteoclastogenic Potential of Tissue-Engineered Periosteal Sheet: Effects of Culture Media on the Ability to Recruit Osteoclast Precursors

• Published in: International journal of molecular sciences Vol. 22; no. 4; p. 2169
• Main Authors: Uematsu, Kohya, Ushiki, Takashi, Ishiguro, Hajime, Ohashi, Riuko, Tamura, Suguru, Watanabe, Mari, Fujimoto, Yoko, Nagata, Masaki, Ajioka, Yoichi, Kawase, Tomoyuki
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.02.2021; MDPI
• Subjects: Adult; Angiogenesis; Animals; Biocompatible Materials; Biomedical materials; Bone marrow; Bone Marrow Transplantation; CD11b; CD11b antigen; Cell culture; Chemokines; Chemotaxis; Collagen; Culture media; Culture Media - pharmacology; Female; Fluorescence; Gamma irradiation; green fluorescent protein; Green Fluorescent Proteins - genetics; Hemopoiesis; Humans; Ligands; Male; Materials Testing; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Transgenic; Osteoclastogenesis; Osteoclasts - cytology; Osteoclasts - drug effects; Osteogenesis; Osteogenesis - drug effects; Osteoprogenitor cells; Periodontium - cytology; periosteal sheets; Phosphatase; Radiation; Sheets; Stem cell transplantation; Stem cells; Surgical implants; tartrate-resistant acid phosphatase; Tissue engineering; Tissue Engineering - methods; Transplantation; Trinucleotide repeats; Young Adult; tartrate-resistant acid phosphatase; osteoclastogenesis; periosteal sheets; green fluorescent protein; CD11b; bone marrow transplantation
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22042169

Cell culture media influence the characteristics of human osteogenic periosteal sheets. We have previously found that a stem cell medium facilitates growth and collagen matrix formation in vitro and osteogenesis in vivo. However, it has not yet been demonstrated which culture medium is superior for osteoclastogenesis, a prerequisite for reconstruction of normal bone metabolic basis. To address this question, we compared chemotaxis and osteoclastogenesis in tissue-engineered periosteal sheets (TPSs) prepared with two types of culture media. Periosteal tissues obtained from adult volunteers were expanded with the conventional Medium 199 or with the stem cell medium, MesenPRO. Hematopoietic enhanced-green-fluorescent-protein (EGFP)-nude mice were prepared by γ-irradiation of Balb/c nu/nu mice and subsequent transplantation of bone marrow cells from CAG-EGFP C57BL/6 mice. TPSs were implanted subcutaneously into the chimeric mice and retrieved after intervals for immunohistopathological examination. EGFP cells were similarly recruited to the implantation site in both the TPSs prepared, whereas the distribution of CD11b cells was significantly lower in the TPS prepared with the stem cell medium. Instead, osteoclastogenesis was higher in the TPS prepared with the stem cell medium than in the one prepared with the conventional medium. These findings suggest that the stem cell medium is preferable for the preparation of more functional TPSs.