In Vivo Detection of Gold Nanoshells in Tumors Using Diffuse Optical Spectroscopy

• Published in: IEEE journal of selected topics in quantum electronics Vol. 13; no. 6; pp. 1715 - 1720
• Main Authors: Zaman, Raiyan T., Diagaradjane, Parmeswaran, Wang, James C., Schwartz, Jon, Rajaram, Narasimhan, Gill-Sharp, Kelly L., Cho, Sang H., Rylander, Henry Grady, Payne, J. Donald, Krishnan, Sunil, Tunnell, James W.
• Format: Journal Article
• Language: English
• Published: United States IEEE 01.11.2007; The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
• Subjects: Biomedical materials; Contrast agents; diffuse optical spectroscopy (DOS); Diffusion; diffusion theory; Gold; gold nanoparticles; In vitro; In vivo; Mice; Nanocomposites; Nanomaterials; Nanostructure; Neoplasms; Noninvasive treatment; Optical fibers; optical imaging; Performance evaluation; Probes; Spectra; Spectroscopy; Surgical implants; Tumors; diffuse optical spectroscopy (DOS); diffusion theory; Contrast agents; gold nanoparticles; optical imaging
• ISSN: 1077-260X; 1558-4542
• DOI: 10.1109/JSTQE.2007.910804

This study demonstrates the use of diffuse optical spectroscopy (DOS) for the noninvasive measurement of gold nanoshell concentrations in tumors of live mice. We measured the diffuse optical spectra (500-800 nm) using an optical fiber probe placed in contact with the tissue surface. We performed in vitro studies on tissue phantoms illustrating an accurate measurement of gold-silica nanoshell concentration within 12.6% of the known concentration. In vivo studies were performed on a mouse xenograft tumor model. DOS spectra were measured at preinjection, immediately postinjection, 1 and 24 h postinjection times, and the nanoshell concentrations were verified using neutron activation analysis.