Cloning and nucleotide sequence of a tobacco chloroplast translational elongation factor, EF-Tu

• Published in: Plant physiology (Bethesda) Vol. 101; no. 1; pp. 333 - 334
• Main Authors: Ursin, V.M. (Calgene Inc., Davis, CA), Becker, C.K, Shewmaker, C.K
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.01.1993
• Subjects: Amino Acid Sequence; AMINO ACID SEQUENCES; Amino acids; Base Sequence; Biological and medical sciences; CHLOROPLASTE; CHLOROPLASTS; Chloroplasts - physiology; CLONACION; CLONAGE; CLONING; Cloning, Molecular; CLOROPLASTO; DNA; elongation factor EF-Tu; Fundamental and applied biological sciences. Psychology; GENBANK/M94204; Genes; Genes, Plant; Genes. Genome; Introns; Molecular and cellular biology; Molecular genetics; MOLECULAR SEQUENCE DATA; Nicotiana - genetics; NICOTIANA TABACUM; NUCLEOTIDE; nucleotide sequence; Nucleotide sequences; NUCLEOTIDES; NUCLEOTIDOS; Open reading frames; Peptide Elongation Factor Tu - genetics; Peptide elongation factors; Plant Gene Register; Plants; Plants, Toxic; predictions; PROTEIN SYNTHESIS; Regional identity; RNA, Messenger - analysis; Sequence Analysis, DNA; SINTESIS DE PROTEINAS; SYNTHESE PROTEIQUE; Gene; Nucleotide sequence; Dicotyledones; Angiospermae; Gene organization; Spermatophyta; Genetical translation; Solanaceae; Molecular cloning; Nicotiana tabacum; Chloroplast; Elongation factor EFTu
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.101.1.333

From a tobacco genomic library, we have cloned a gene encoding tobacco EF-Tu (NtEFTu) utilizing sequence information from the Arabidopsis homolog ATHTufA. The genomic fragment cloned contains an open reading frame of 1444 bp encoding a 478-amino acid protein. The deduced polypeptide contains a 69-amino acid serine-rich chloroplast transit peptide, and the predicted molecular mass of the mature protein is approximately 44.6 kD. Sequence identify between the mature Arabidopsis and tobacco genes is high along their entire coding regions, with complete conservation observed within a 30-amino acid region flanking cysteine-151 (at position 81 in E. gracilis ) that is required for the interaction of the protein with aminoacyl tRNA.