In vitro inhibition of human P450 enzymes by prenylated flavonoids from hops, Humulus lupulus

• Published in: Xenobiotica Vol. 30; no. 3; pp. 235 - 251
• Main Authors: Henderson, M. C., Miranda, C. L., Stevens, J. F., Deinzer, M. L., Buhler, D. R.
• Format: Journal Article
• Language: English
• Published: London Informa UK Ltd 2000; Taylor & Francis
• Subjects: 7-ethoxyresorufin o-deethylase; 8-prenylnaringenin; acetanilide 4-hydroxylase; Aflatoxin B1 - metabolism; aflatoxins; Anticarcinogenic Agents; Aryl Hydrocarbon Hydroxylases - antagonists & inhibitors; Aryl Hydrocarbon Hydroxylases - metabolism; binding; Biological and medical sciences; chalcone; Chemoprevention; Chlorzoxazone - metabolism; cytochrome P-450; Cytochrome P-450 CYP1A1 - antagonists & inhibitors; Cytochrome P-450 CYP3A; Cytochrome P-450 Enzyme Inhibitors; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; cytochrome P450; DNA, Complementary - metabolism; enzyme inhibitors; Flavanones; flavonoids; Flavonoids - pharmacology; General pharmacology; hops; Humans; Humulus lupulus; inhibition; isoxanthohumol; Kinetics; Medical sciences; Microsomes - enzymology; Mixed Function Oxygenases - metabolism; Models, Chemical; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Propiophenones - pharmacology; Protein Binding; Protein Prenylation; Rosales - chemistry; unspecific monooxygenase; xanthohumol; Human; Isozyme; Cytochrome P450; Enzyme inhibitor; Cannabidaceae; Anticarcinogen; Flavonoid; In vitro; Biological activity; Chalcone; Medicinal plant; Dicotyledones; Angiospermae; Humulus lupulus; Plant origin; Spermatophyta
• ISSN: 0049-8254; 1366-5928
• DOI: 10.1080/004982500237631

1. Several unique flavonoid compounds have recently been isolated from hops, Humulus lupulus, and their presence has been detected in beer. Their chemical structures are similar to other plant-derived compounds, many present in the human diet, that have been shown to have cancer chemopreventive properties due, in part, to inhibition of cytochrome P450 enzymes that activate carcinogens. Additionally, preliminary studies have shown these flavonoids (at 100 muM) to be inhibitory of P450-mediated activation reactions in a variety of in vitro systems. Thus, the in vitro effects of these phytochemicals on cDNA-expressed human CYP1A1, CYP1B1, CYP1A2, CYP3A4 and CYP2E1 were currently examined by the use of diagnostic substrates and the carcinogen AFB1. 2. At 10 muM, the prenylated chalcone, xanthohumol (XN), almost completely inhibited the 7-ethoxyresorufin O-deethylase (EROD) activity of CYP1A1. At the same concentration, other hop flavonoids decreased the EROD activity by 90.8-27.0%. 3. At 10 muM, XN completely eliminated CYP1B1 EROD activity, whereas the other hop flavonoids showed varying degrees of inhibitory action ranging from 99.3 to 1.8%. 4. In contrast, the most effective inhibitors of CYP1A2 acetanilide 4-hydroxylase activitywere the two prenylated flavonoids, 8-prenylnaringenin (8PN) and isoxanthohumol (IX), which produced > 90% inhibition when added at concentrations of 10 mu M. 5. CYP1A2 metabolism of the carcinogen AFB1 was also inhibited by IX and 8PN as shown by decreased appearance of dihydrodiols and AFM1 as analysed by hplc. IX and 8PN also decreased covalent binding of radiolabelled AFB1 to microsomal protein in a concomitant manner. 6. XN, IX and 8PN, however, were poor inhibitors of CYP2E1 and CYP3A4 as measured by their effect on chorzoxazone hydroxylase and nifedipine oxidase activities respectively. 7. These results suggest that the hop flavonoids are potent and selective inhibitors of human cytochrome P450 and warrant further in vivo investigations.