RNA-interference-based functional genomics in mammalian cells: reverse genetics coming of age

Sequencing of complete genomes has provided researchers with a wealth of information to study genome organization, genetic instability, and polymorphisms, as well as a knowledge of all potentially expressed genes. The identification of all genes encoded in the human genome opens the door for large-s...

Full description

Saved in:
Bibliographic Details
Published inOncogene Vol. 23; no. 51; pp. 8401 - 8409
Main Authors Silva, Jose, Chang, Kenneth, Hannon, Gregory J, Rivas, Fabiola V
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 01.11.2004
Subjects
Animals
Apoptosis
Cancer therapies
Cell division
Gene Library
Gene silencing
Genes
Genomes
Genomic instability
Genomics
Humans
Mammalian cells
RNA Interference - physiology
RNA-mediated interference
siRNA
Transformed cells
Online AccessGet full text

Cover

More Information
Summary:Sequencing of complete genomes has provided researchers with a wealth of information to study genome organization, genetic instability, and polymorphisms, as well as a knowledge of all potentially expressed genes. The identification of all genes encoded in the human genome opens the door for large-scale systematic gene silencing using small interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs). With the recent development of siRNA and shRNA expression libraries, the application of RNAi technology to assign function to cancer genes and to delineate molecular pathways in which these genes affect in normal and transformed cells, will contribute significantly to the knowledge necessary to develop new and also improve existing cancer therapy.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-3
ObjectType-Review-2
ISSN:0950-9232
1476-5594
DOI:10.1038/sj.onc.1208176