Role of Plasma Membrane Calcium ATPases in Calcium Clearance from Olfactory Sensory Neurons

• Published in: Chemical senses Vol. 34; no. 4; pp. 349 - 358
• Main Authors: Saidu, S. Ponissery, Weeraratne, S.D., Valentine, M., Delay, R., Van Houten, Judith L.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.05.2009; Oxford Publishing Limited (England)
• Subjects: Animals; Biological and medical sciences; calcium; Calcium - metabolism; Cell Membrane - enzymology; Cells, Cultured; Colforsin - pharmacology; Enzyme Inhibitors - pharmacology; Fundamental and applied biological sciences. Psychology; Gene Knockout Techniques; Indoles - pharmacology; Kinetics; Mice; Mice, Inbred C57BL; Mice, Knockout; mouse; Olfaction. Taste; olfactory neurons; Olfactory Receptor Neurons - drug effects; Olfactory Receptor Neurons - enzymology; Perception; Plasma Membrane Calcium-Transporting ATPases - metabolism; PMCA; Protein Isoforms - metabolism; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; pumps; Sodium - metabolism; Sodium-Calcium Exchanger - metabolism; mouse; pumps; calcium; kinetics; PMCA; olfactory neurons; Calcium; Rodentia; Clearance; Vertebrata; Mammalia; Mouse; Animal; Plasma membrane; Olfaction; Perception; Sensory neuron; Kinetics
• ISSN: 0379-864X; 1464-3553
• DOI: 10.1093/chemse/bjp008

Odorants cause Ca2+ to rise in olfactory sensory neurons (OSNs) first within the ciliary compartment, then in the dendritic knob, and finally in the cell body. Ca2+ not only excites but also produces negative feedback on the transduction pathway. To relieve this Ca2+-dependent adaptation, Ca2+ must be cleared from the cilia and dendritic knob by mechanisms that are not well understood. This work focuses on the roles of plasma membrane calcium pumps (PMCAs) through the use of inhibitors and mice missing 1 of the 4 PMCA isoforms (PMCA2). We demonstrate a significant contribution of PMCAs in addition to contributions of the Na+/Ca2+ exchanger and endoplasmic reticulum (ER) calcium pump to the rate of calcium clearance after OSN stimulation. PMCAs in neurons can shape the Ca2+ signal. We discuss the contributions of the specific PMCA isoforms to the shape of the Ca2+ transient that controls signaling and adaptation in OSNs.