Altered Gene Expression of RNF34 and PACAP Possibly Involved in Mechanism of Exercise-Induced Analgesia for Neuropathic Pain in Rats

• Published in: International journal of molecular sciences Vol. 18; no. 9; p. 1962
• Main Authors: Yamaoka, Shintaro, Oshima, Yusuke, Horiuchi, Hideki, Morino, Tadao, Hino, Masayuki, Miura, Hiromasa, Ogata, Tadanori
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13.09.2017; MDPI
• Subjects: Analgesia; Analgesia - methods; Animals; Carrier Proteins - biosynthesis; Carrier Proteins - genetics; Chronic pain; Disease Models, Animal; Dorsal horn; Exercise Test; exercise therapy; Female; Gene expression; Gene Expression Regulation; Hyperalgesia; LMD; Neuralgia - genetics; Neuralgia - metabolism; Neuralgia - therapy; neuropathic pain; PACAP; Pain; Pain Management - methods; Pain perception; Pharmacology; Physical Conditioning, Animal; Pituitary adenylate cyclase-activating polypeptide; Pituitary Adenylate Cyclase-Activating Polypeptide - biosynthesis; Pituitary Adenylate Cyclase-Activating Polypeptide - genetics; Rats; RNA sequence; RNA, Messenger - genetics; RNA, Messenger - metabolism; RNF34; Rodents; SNL model; Spinal cord; Spinal Cord - metabolism; Spinal Cord - physiopathology; Spinal Cord Dorsal Horn - metabolism; Spinal Cord Injuries - metabolism; Spinal Cord Injuries - pathology; Spinal Cord Injuries - physiopathology; Spinal Nerves - surgery; Surgery; LMD; neuropathic pain; RNF34; exercise therapy; PACAP; RNA sequence; SNL model
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms18091962

Despite the availability of several modalities of treatment, including surgery, pharmacological agents, and nerve blocks, neuropathic pain is often unresponsive and sometimes progresses to intractable chronic pain. Although exercise therapy is a candidate for treatment of neuropathic pain, the mechanism underlying its efficacy has not been elucidated. To clarify the molecular mechanism for pain relief induced by exercise, we measured and mRNA levels in the spinal cord dorsal horn of SNL rats, a model of neuropathic pain. SNL model rats exhibited stable mechanical hyperalgesia for at least 6 weeks. When the rats were forced to exercise on a treadmill, mechanical and thermal hyperalgesia were significantly ameliorated compared with the non-exercise group. Accordingly, gene expression level of and were also significantly altered in the time course analysis after surgery. These results suggest that exercise therapy possibly involves pain relief in SNL rats by suppressing and expression in the spinal cord.