Sodium Polyanethol Sulfonate Modulates Natural Transformation of SigH-Expressing Staphylococcus aureus

• Published in: Current microbiology Vol. 75; no. 4; pp. 499 - 504
• Main Authors: 大庭 良介, 齋藤 慎二, 森川 一也, Nguyen Le Thuy Thi, Takemura Aya J., Ohniwa Ryosuke L., Saito Shinji, Morikawa Kazuya
• Format: Journal Article
• Language: English
• Published: New York Springer 01.04.2018; Springer US; Springer Nature B.V
• Subjects: Autolysis; Biomedical and Life Sciences; Biotechnology; carbon disulfide; Cell culture; Cell walls; Cytology; Deoxyribonucleic acid; Disintegration; DNA; dose response; Gene expression; Genetic transformation; Life Sciences; Metabolism; Microbiology; Sigma factor; Sodium; Staphylococcus aureus; Staphylococcus infections; Sulfonates; transcription (genetics)
• ISSN: 0343-8651; 1432-0991; 1432-0991
• DOI: 10.1007/s00284-017-1409-5

Expression of genes required for natural genetic competence in Staphylococcus aureus is controlled by an alternative transcription sigma factor, SigH. However, even in the SigH-expressing cells, the DNA transformation efficiency varies depending on culture conditions. We report here that cells grown in the competence-inducing medium (CS2 medium) exhibit enlarged morphology with disintegrated cell walls. Notably, an autolysis inhibitor, Sodium Polyanethol Sulfonate (SPS), facilitated transformation in CS2 medium in a dose-dependent manner, suggesting the involvement of the cell wall metabolism in transformation. However, the transformation efficiency of cells grown in TSB was not improved by physical or enzymatic damage on the cell walls.